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  • 1970-1974  (7)
Material
Years
Year
  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Anaesthesia 28 (1973), S. 0 
    ISSN: 1365-2044
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 2
    facet.materialart.
    Unknown
    London, etc. : Periodicals Archive Online (PAO)
    Slavonic and East European review. 51:122 (1973:Jan.) 151 
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  • 3
    facet.materialart.
    Unknown
    London, etc. : Periodicals Archive Online (PAO)
    Slavonic and East European review. 49:115 (1971:Apr.) 323 
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Planta 117 (1974), S. 163-172 
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Anacystis nidulans will take up and accumulate chloride ions. When the external concentration was 0.2 mM Cl- the level in the cells was 2.8 mM Cl- and under these conditions the flux across the cell surface was in the region of 10-13equiv Cl-·sec-1·cm-2. It is suggested that this Cl- influx is active and operates against an electrochemical potential gradient estimated to be 117 mV or 2.68 kcal/mole. The uptake of 36Cl was inhibited by low temperatures and there was a net loss of Cl- from the cells with the level decreasing towards the equilibrium value as estimated from K+ distribution. Although the active influx of Cl- was often stimulated by light this was not always the case. Dark storage treatment and regulation of the chlorophyll a/phycocyanin ratios as well as total pigment content of the cells did not clarify the conditions which brought about light stimulation. Moreover, the metabolic inhibitors CCCP and CMU and also the use of anaerobic conditions did not clearly indicate the relationship between the influx mechanism and light-dark metabolism and no firm conclusions could be made about the nature of the energy source. The variation in the degree of light stimulation probably reflects the fact that in this procaryotic organism the photosynthetic and respiratory units are located on the same membrane systems and are in very close proximity to the probable site of the Cl- pump, the plasmalemma.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Planta 109 (1973), S. 49-60 
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Addition of mercuric chloride at concentrations which resulted in an overall binding level of about 8 mmoles Hg/l packed cells and above caused a breakdown in the permeability of the cell membrane as indicated by a net efflux of internal K+. Below this level in region of 2 mmoles Hg/l packed cells the rate of K+ transfer across the cell surface was stimulated without affecting the internal K+ level. Maintainence of the stimulation was dependent both on time and dose. Enhancement of the rate of K+ turnover was associated with a fast component of the inorganic mercury uptake which could be removed by washing with cysteine. The mercury stimulated K+/K+ exchange was inhibited by low temperature, by the uncoupler CCCP and the energy transfer inhibitor DCCD. Overall binding concentrations of inorganic mercury below 0.5 mmoles/l packed cells had no effect on the K+ transport system. In contrast to mercuric chloride, methyl mercuric chloride over similar concentration ranges did not seem to induce a breakdown in the permeability barrier or directly interact with the K+/K+ exchange but more likely influenced the latter by inhibiting intracellular processes.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Planta 111 (1973), S. 13-22 
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The rate of Na+/Na+ exchange as measured with 24Na+ in Na+-rich cells of Chlorella pyrenoidosa is governed by a single rate constant and saturates with increasing external Na+ concentration. The K mvalue for this process is 0.8 mM Na+ and the maximum rate of exchange in illuminated cells is about 5 pmoles cm-2 sec-1. These values contrast with a K mof 0.18 mM K+ and maximum rate of about 17 pmoles K+·cm-2·sec-1 for net K+ influx. Although the Na+/Na+ exchange was only slightly sensitive to light it was inhibited by the uncouplers CCCP and DNP and by the energy transfer inhibitor DCCD. This inhibition of the rate of Na+/Na+ exchange was not accompanied by a loss of internal Na+. Both the effect of external K+ on 24Na+ influx into Na+-rich cells and the inhibition of net K+ uptake by the presence of external Na+ indicates that Na+/Na+ and K+/Na+ exchanges share the same carrier and that the external site of this carrier has a three to four times higher affinity for K+ over Na+.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Planta 113 (1973), S. 143-155 
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Anacystis nidulans accumulates K+ in preference to Na+. The majority of the internal K+ exchanges with 42K by a first order process at rates of about 1.3 pequiv·cm-2·sec-1 in the light and 0.26 pequiv·cm-2·sec-1 in the dark. Although the K+/K+ exchange was stimulated by light and inhibited by 10-4 M CCCP and 10-5 M DCMU there are several indications that this cation is passively distributed in Anacystis. Inhibition of the exchange by CCCP and DCMU occurred at concentrations greater than those required to inhibit photosynthesis and the K+ fluxes were stimulated by low temperatures. Moreover, although valinomycin stimulated the exchange this compound did not induce a net K+ leak. Assuming K+ is passively distributed and in free solution within the cytoplasm, as indicated by osmotic studies, would imply that there is an active Na+ extrusion pump operating in this organism. As yet there are no firm conclusions about the nature of the energy source for this efflux pump.
    Type of Medium: Electronic Resource
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