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  • 1970-1974  (2)
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Chromosoma 48 (1974), S. 203-211 
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The distribution of gaps, breaks, exchanges and other effects induced by Mitomycin C on human chromosomes was analysed to examine the possibility of a correlation between chromosome lesions and regions of repetitive DNA. Homologous and non-homologous exchanges between chromosomes Nos. 1, 9 and 16, and also between and with the acrocentric chromosomes were more frequent than expected, but breaks and gaps appeared to be located randomly with regard to chromosome light and dark bands.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-6857
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: This report describes a method for the direct preparation of chromosomes from the hepatocytes and bone marrow of the same Chinese hamster (Cricetulus griseus). The technique is a modification of that described by Becker et al. (J. natl. Ca. Inst. 46: 1261–69, 1971) for rat hepatocytes, with the following significant differences: (1) a less extensive partial hepatectomy is employed to initiate hepatocyte regeneration, (2) the use of a larger initial dose of colchicine (4 mg/K) 46–48 hours after hepatectomy instead of 1 mg/K 24 hours after hepatectomy, (3) the use of 0.075 M KCl as hypotonic solution instead of fetal calf serum diluted 1 : 7 with distilled water and (4) flame or blaze drying of chromosome preparations instead of air drying. The combination of the above modifications gave abundant, clear and well-spread chromosomes.
    Type of Medium: Electronic Resource
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