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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 101 (1974), S. 153-159 
    ISSN: 1432-072X
    Keywords: Azotobacter vinelandii ; Nitrogenase ; Repression ; Ammonia Determination ; Oxygen Effect
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A method is described which allows the quantitative determination of small ammonia concentrations in the culture of nitrogen-fixing microorganisms. With this method the ammonia concentration range was estimated in which repression of nitrogenase synthesis in Azotobacter vinelandii occurs. Both in batch and continuous cultures there was no repression below 10 μM, whereas nitrogenase synthesis stopped completely if the ammonia concentration in the medium exceeded 25 μM.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 98 (1974), S. 93-100 
    ISSN: 1432-072X
    Keywords: Azotobacter ; Nitrogenase ; Iron Sulfur Proteins ; Properties
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The purified Mo-Fe protein and Fe protein of nitrogenase from Azotobacter vinelandii have molecular weights (MW) of about 216000 and 64000, respectively. The Mo-Fe protein is composed of subunits of about 56000 MW, and the Fe protein has 2 equivalent subunits of about 33000 MW. The isoelectric point of the Mo-Fe protein is 5.2 and that of the Fe protein is 4.7. Amino acid compositions reflect the acidic nature of the proteins. The Mo-Fe protein yielded 24 atoms of Fe, 20 atoms of acid-labile sulfide and 1.54 atoms of Mo per molecule. Analysis of the Fe protein showed 3.45 atoms of Fe per molecule.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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