ISSN:
0006-3592
Schlagwort(e):
Chemistry
;
Biochemistry and Biotechnology
Quelle:
Wiley InterScience Backfile Collection 1832-2000
Thema:
Biologie
,
Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
Notizen:
A general model of the kinetics of microbial growth has been developed involving the kinetics of incorporation of substrate into biomass and the maintenance energy requirements.Results obtained from batch cultures of the yeast Saccharomyces cerevisiae growing in synthetic media at pH 5.1 and 30°C permitted all biological parameters in the model to be calculated.Values obtained for these parameters were: maximum specific glucose uptake rate (μSm), 2.08 g/g biomass/hr; apparent Michaelis constant for glucose (KS), 0.1 g/liter (5.5 × 10-4M) apparent Michaelis constant for oxygen (KL), 1.4% O2 (3.2 × 10-6 M) quantitative index of the Pasteur effect (b), 4.9 × 10-4%-1 O2 (207 M -1). Under conditions of strongly substrate-repressed respiration the values obtained for YATP and P/O were constant over the course of the exponential phase of growth (YATP = 10.4 g biomass/mole ATP; P/O = 3 moles ATP/atom 0). Mass balances for aerobic and anaerobic cultures confirmed the results obtained form the generalized model. Results presented suggested the operation of a mechanism for regulating energy-yielding metabolism which involved an equilibrium between the systems of oxidative phosphorylation and dephosphorylation and was dependent upon the level of catbolite repression.
Zusätzliches Material:
8 Ill.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1002/bit.260160402
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