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  • 1970-1974  (8)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    The journal of membrane biology 3 (1970), S. 400-401 
    ISSN: 1432-1424
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    [S.l.] : International Union of Crystallography (IUCr)
    Acta crystallographica 28 (1972), S. 440-444 
    ISSN: 1600-5724
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: The most time-consuming step in the analytical method for absorption correction is the examination for each Howells polyhedron of the large number of tetrahedra formed from all possible sets of one auxiliary point with the faces of previously found tetrahedra. In the present method, a formula is presented for the absorption of a polyhedron (a slice) with two parallel faces, which are planes of constant absorption. With this formula, the absorption of a Howells polyhedron can be calculated, with much less effort, by systematically dividing it into slices.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    British journal of dermatology 90 (1974), S. 0 
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: A method is presented for simultaneous in vitro growth and measurement of permeability of human skin. During culture of specimens, the parakeratotic layer formed is shown to possess considerable barrier function. Maximum barrier capacity of this layer in vitro is attained at 14–17 days’ incubation. When the stratum corneum is removed prior to incubation, the maximum barrier capacity is reached in 3–4 days. The difference in behaviour between intact and stripped skin is reflected in the pattern of DNA synthesis of the basal cells, which show an enhanced proliferation after stripping. Barrier regeneration of stripped skin is seen closely to resemble the parallel situation in vivo.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    British journal of dermatology 86 (1972), S. 0 
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Summary.— In this report we investigate the role of serum in organ culture of adult human skin. The migratory overgrowth of dermis by epidermal cells is shown to depend on the presence of high molecular weight (mol. wt.) components of serum. Regeneration of the epidermis after 19 days' incubation occurs in the presence of 10% whole serum in the growth medium. DNA synthesis is found to increase in long-term incubations in medium including serum components but not in the presence of Minimal Essential Medium (MEM) only.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 8 (1973), S. 0 
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: It is now generally accepted that chronic periodontitis probably follows the accumulation of bacterial plaque on teeth surfaces. Some investigators have claimed to show that substances from plaque damage cells in vitro, but their results appear difficult to assess. The present investigation was a further attempt at examining the effect of substances in an aqueous plaque extract on mammalian cell cultures in vitro. In addition, this effect was compared with that caused by substances in saliva. Plaque was collected onto ice from patients requiring dental treatment. The plaque was pooled, and homogenised in carrier saline (Earle's saline from which NaH2PO4, NaHCO3, and glucose had been omitted). The mixture was homogenised at 4°C. centrifuged, and the supernatant fraction, the plaque extract (PE), sterilised by millipore filtration. Unstimulated whole saliva was centrifuged, and the supernatant fraction sterilised in the same way as the Pe # HeLa, L 929, and BHK 21 C 13 established cell cultures, and foetal human lung fibroblasts at 4th passage in cell culture, were grown in Eagle's medium with Earle's saline, calf serum to 10% v/v, and penicillin and streptomycin each to 100 units per ml. The PE was found to contain material which damaged HeLa cells, and prevented the growth of all 4 cell types. This material did not seem to detach any of these cells from the dish, and was 20 times more concentrated in plaque than in saliva. These results indicate that mammalian cell cultures can be used to detect toxic substances in aqueous plaque extracts. These substances may possibly irritate junctional epithelium at the base of the gingival sulcus or pocket in vivo.
    Type of Medium: Electronic Resource
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  • 6
    facet.materialart.
    Unknown
    Baltimore, Md. : Periodicals Archive Online (PAO)
    Behavioral science. 19:2 (1974:Mar.) 79 
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  • 7
    facet.materialart.
    Unknown
    Baltimore, Md. : Periodicals Archive Online (PAO)
    Behavioral science. 19:6 (1974:Nov.) 407 
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Neuroradiology 5 (1973), S. 1-6 
    ISSN: 1432-1920
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé L'auteur a étudié les modifications du liquide céphalo-rachidien (L.C.R.) chez 165 malades soumis à une encéphalographie gazeuse par voie lombaire. Des modifications importantes ne survenaient que dans la numération cellulaire et la concentralion protéinique. Dans 60% des cas apparut une pléocytose lymphocytaire prédominante, supérieure à deux cellules par mm3. La réaction cellulaire était proportionnelle au temps séparant les prélèvements initial et final de LCR, plus grande lorsque la numération des leucocytes initiale excèdait cinq cellules par mm3 et/ou en rapport avec une réaction inflammatoire intracrânienne. On relevait une diminution relativement constante d'environ 20% de la teneur en protéines du LCR. Cette diminution semblait due essentiellement à la dilution partielle du LCR lombaire avec le liquide ventriculaire et cisternal. Compte-tenu des grandes variations individuelles dans la teneur en protéines, en particulier dans les cas limites, il est recommandé de n'utiliser que le prélèvement initial de LCR pour les examens biochimiques et l'analyse cellulaire afin d'eviter toute erreur de diagnostic.
    Abstract: Zusammenfassung Es handelt sich um Untersuchungen von 165 Patienten, bei denen eine Luftencephalographie durchgeführt wurde. Signifikante Veränderungen zeigten sich lediglich in der Zellzahl und im Eiweißgehalt. Aus der Arbeit geht eindeutig hervor, daß nur der zuerst gewonnene Liquor nach der Lumbalpunktion für biochemische und Zellanalysen verwandt werden kann. Ausführliche Beschreibung der gefundenen Veränderungen.
    Notes: Summary The changes in the cerebrospinal fluid (CSF) during lumbar air encephalography were studied in 165 patients. Significant changes occurred only in the cell count and protein concentration. A predominantly lymphocytic pleocytosis of greater than 2 cells per cu mm appeared in 60% of cases, the cellular reaction being proportional to the time between initial and final CSF samples, and greater when the initial CSF white cell count exceeded 5 cells/mm3 and/or in disorders associated with an intracranial inflammatory reaction. A relatively constant decrease in the total CSF protein content of approximately 20% of the initial value was noted, and was felt to be due primarily to partial dilution of the lumbar CSF by ventricular and cisternal fluid.—In view of the wide individual variations in the changes in protein content, particularly in the borderline abnormal range, it is recommended that only the initial CSF specimen be used for biochemical and cellular analysis, to avoid diagnostic confusion.
    Type of Medium: Electronic Resource
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