ISSN:
1399-3054
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
Addition of octanoic acid (2· 10-3M) to the suspending medium (final pH 4.85) of Boletus variegatus mycelium induced a marked leaking of UV absorbing substances from the cells. The material had an absorption maximum at 260 nm, a minimum at 240 nm, and the absorption ratios 250: 260 and 280:260 were 0.81 and 0.49.The material released immediately after addition of the acid consisted mainly of low molecular weight substances. These substances, listed according to decreasing rates of leaking, were identified as pentoses, pentosephosphates, nucleosides, and mono- and di-nucleotides. Also, purine and pyrimidine bases were released at this early stage of treatment.After 90 minutes' treatment, an outflux of oligoribonucleotides was observed. The oligoribonucleotides did not occur as single substances, but were forming complexes with peptides. Minor amounts of ribonucleic acid were also leaking out from the cells. Deoxyribose containing substances were never observed in the filtrates.The compounds were subjected to enzymatic degradation after they had left the cells. This was shown by a marked increase with time of inorganic phosphorus, pentose/pentosephosphates, and nucleosides in the filtrate.The leaking of low molecular weight substances immediately after acid addition is correlated to seriously reduced growth. However, the growth was wholly restored after a three days' lag period. On the other hand, when considerable amounts of oligoribonucleotide peptides had been released from the cells, growth could not be re-established.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1399-3054.1970.tb06466.x
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