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  • 1970-1974  (3)
  • 1
    Electronic Resource
    Electronic Resource
    Phospholipase-C from Bacillus cereus: Production, purification, and properties (1973)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 15 (1973), S. 551-560 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A substrain of Bacillus cereus 569/H produced under controlled fermentation conditions in a pilot plant fermentor phospholipase-C. A partially purified preparation showed good storage stability as a lyophylized powder and in frozen solutions. The preparation contained very small amounts of phosphomonoesterase and proteolytic activities and essentially no ribonuclease activity. The level of hemolytic activity of the preparation was much lower than that of a commercial preparation of phospholipase-C from Clostridium. Treatment of sarcoplasmic reticulum membrane with phospholipase-C from B. cereus and from Clostridium showed that the B. cereus enzyme caused hydrolysis of 96% of the membrane phospholipids whereas the enzyme from Clostridium could hydrolyze only 80% of the phospholipids.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260150310
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    A modified procedure for the large scale preparation of tRNA from E. coli (1973)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 15 (1973), S. 1081-1088 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A procedure for the preparation of about 50 g batches of tRNA from 25 kg E. coli W is described. The method involves phenolic extraction of the cells, batch absorption of the tRNA on DEAE-cellulose, washing the DEAE-cellulose and packing it into a column, elution of the tRNA from the column and precipitation of the tRNA with ethanol. The method is less time and labor consuming than the methods described in the literature and can be carried out with relatively simple equipment.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260150607
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Maximal exponential growth rate and yield of E. coli obtainable in a bench-scale fermentor (1974)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 16 (1974), S. 933-941 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Oxygen supply is one of the main factors which influences aerobic cell growth in a fermentor. Maximal rates at which E. coli can grow on glucose as carbon source under various limiting oxygen-supply conditions were determined in a bench-scale fermentor. Culture conditions are described which gave yields of about 38 g dry cells per liter medium.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260160707
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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