Library

feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • 1970-1974  (3)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 79 (1971), S. 164-175 
    ISSN: 1432-072X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Enzymatic and genetic evidence are presented for a new pathway of ammonia assimilation in nitrogen fixing bacteria: ammonium → glutamine → glutamate. This route to the important glutamate-glutamine family of amino acids differs from the conventional pathway, ammonium → glutamate → glutamine, in several respects. Glutamate synthetase [(glutamine amide-2-oxoglutarate aminotransferase) (oxidoreductase)], which is clearly distinct from glutamate dehydrogenase, catalyzes the reduced pyridine nucleotide dependent amination of α-ketoglutarate with glutamine as amino donor yielding two molecules of glutamate as product. The enzyme is completely inhibited by the glutamine analogue DON, whereas glutamate dehydrogenase is not affected by this inhibitor; the glutamate synthetase reaction is irreversible. Glutamate synthetase is widely distributed in bacteria; the pyridine nucleotide coenzyme specificity of the enzyme varies in many of these species. The activities of key enzymes are modulated by environmental nitrogenous sources; for example, extracts of N2-grown cells of Klebsiella pneumoniae form glutamate almost exclusively by this new route and contain only trace amounts of glutamate dehydrogenase activity whereas NH3-grown cells possess both pathways. Also, the biosynthetically active form of glutamine synthetase with a low K m for ammonium predominates in the N2-grown cell. Several mutant strains of K. pneumoniae have been isolated which fail to fix nitrogen or to grow in an ammonium limited environment. Extracts of these strains prepared from cells grown on higher levels of ammonium have low levels of glutamate synthetase activity and contain the biosynthetically inactive species of glutamine synthetase along with high levels of glutamate dehydrogenase. These mutants missing the new assimilatory pathway have serious defects in their metabolism of many inorganic and organic nitrogen sources; utilization of at least 20 different compounds is effected. We conclude that the new ammonia assimilatory route plays an important role in nitrogenous metabolism and is essential for nitrogen fixation.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 2
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Résumé Le PF-257 ou chlorhydrate de benzisoxalzole-1,2 acétamidoxime-3 produit des effets variés sur le système nerveux central des animaux. Le profil pharmacologique et l'action fondamentale de cet agent paraissent nouveaux, comparés à ceux des psychotropiques actuels.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 3
    Electronic Resource
    Electronic Resource
    New York : Wiley-Blackwell
    Journal of Polymer Science: Polymer Physics Edition 12 (1974), S. 1189-1193 
    ISSN: 0098-1273
    Keywords: Physics ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: The kinetics of the disappearance of propagating butadiene radicals produced in the grafting of butadiene to poly(vinyl chloride) was followed by electron spin resonance measurements. The radical species observed in the temperature range 213-298°K is the conjugated allylic radical: \documentclass{article}\pagestyle{empty}\begin{document}$$ \hbox{---} {\rm CH}_{2}\hbox{---}\rm{CH}\hbox{=\hskip-1pt=}{\rm CH}\hbox{---}{\rm CH}_2 \rightleftharpoons\hbox{---}{\rm CH}_2\hbox{---}{\rm CH}\hbox{---}{\rm CH}\hbox{=\hskip-1pt=}{\rm CH}_2 $$\end{document} The reaction conditions were adjusted to provide a highly viscous medium in which the propagation reaction took place. Under these conditions the radicals were stabilized and a precise ESR spectrum was obtained. Arrhenius plots of the second-order decay rate constants yield an activation energy of 5 kcal/mole. This suggests that the bimolecular termination reaction is controlled by the segmental motion of the grafted side chains.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...