ISSN:
1432-8798
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Poliovirus inhibitors present in normal equine and bovine sera were studied by means of radioimmunoelectrophoresis and column chromatography. The inhibitors in individual equine sera were identified as either one of the three immunoglobulins, γM, γA(T) and γG, or mixtures of them, while those in bovine sera were found to be either one of γM and γG globulins, or the mixture of the two. The inhibitors belonging to different classes of immunoglobulin differed in their mode of action against the virus. γM inhibitors were characterized by distinct activities in the kinetic neutralization and the plaque reduction tests. In contrast, γA(T) inhibitors were highly active in the plaque-reduction and the precipitation-in-agar-gel tests, although they were inactive in the kinetics of neutralization. There were two types of γG inhibitors. One did not neutralize the virus in the kinetic test, despite its distinct virus-precipitating activity. The other could neutralize but not precipitate the virusin vitro. Avidity of the inhibitors as revealed by dissociation of virus-inhibitor complexes at acidic pH's also differed among the immunoglobulin classes of the inhibitors. The complex formed with the NaIO4-treated γA(T) inhibitor was most stable and was not dissociated even at pH as low as 2.0.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01254169
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