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  • 1
    Electronic Resource
    Electronic Resource
    Hydrocarbons, free fatty acids, and amino acids of sclerotia of Sclerotinia sclerotiorum (1970)
    Springer
    Archives of microbiology 75 (1970), S. 59-66 
    Details
    ISSN: 1432-072X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Sclerotia of Sclerotinia sclerotiorum (Lib.) D By. were obtained from commercial pea-and bean-cleaning operations or grown on potato-dextrose agar and synthetic glucose-and sucrose-salts agar media. The crude fat (ether extract) content of sclerotia varied from 0.8 to 1.5%. Extraction and fractionation of the lipids followed by gas chromatographic analysis showed that sclerotia from pea cleanings contained one predominant hydrocarbon which was absent from sclerotia produced in the laboratory. Sclerotia from natural sources and grown in the laboratory contained a similar distribution of C18 unsaturated free fatty acids, however, quantitative differences were noted. Palmitic, oleic and linoleic were the major free fatty acids of the laboratory-grown sclerotia while a high proportion of linoleic acid was also found in sclerotia from natural sources. Sclerotia were fractionated into water-soluble and water-insoluble fractions. After acid hydrolysis of the waterinsoluble fraction, both fractions were analyzed for amino acids. Twenty-one compounds, including 2 unknowns, were detected in the soluble fraction. The hydrolyzates contained 19 amino acids, including the same 2 unknowns. Two compounds tentatively identified as ornithine and γ-aminobutyric acid were found only in the water-soluble fraction. The relative amino acid composition of the water-insoluble fraction of sclerotia from various sources was fairly constant but the arginine content decreased on the synthetic media.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00412093
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  • 2
    Electronic Resource
    Electronic Resource
    Effect of dimethyl sulphoxide on growth, sporulation and spore germination ofRhizopus arrhizus Fischer (1972)
    Springer
    Archives of microbiology 82 (1972), S. 184-188 
    Details
    ISSN: 1432-072X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Influences of various levels of dimethyl sulphoxide (DMSO) ranging from 0.04–1.28 M on growth, sporulation and spore germination ofRhizopus arrhizus in liquid and semi-solid media were investigated. Light enhanced the inhibitory effect of DMSO on growth and sporulation in both media. Inhibition of spore germination was observed with direct treatment with DMSO. The inhibition was higher in spores obtained from cultures grown on the DMSO semi-solid media.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01890409
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  • 3
    Electronic Resource
    Electronic Resource
    The Influence of Salinity and Temperature on Seed Germination in Salicornia bigelovii (1971)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 24 (1971), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Seeds of Salicornia bigelovii were germinated at 4.4°C, 15.5°C, and 26.6°C in saline solution containing from 0% to 8.08% sea salt. At 4.4°C, germination was delayed until the 26th day, but the final germination per cent was high in all salinities. At 15.5°C, germination was delayed until the 19th day, and the germination per cent was higher in the higher salinities. At 26.6°C, the germination began within one day and the germination per cent was higher at the lower salinities. With the exception of 26.6°C data, the maximum germination occurred at a sea salt concentration at 4.04 % which is very close to the salinity of the sea.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1971.tb06719.x
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  • 4
    Electronic Resource
    Electronic Resource
    Ultrastructure of dormant and germinated sporangiospores ofRhizopus arrhizus (1973)
    Springer
    Protoplasma 77 (1973), S. 15-33 
    Details
    ISSN: 1615-6102
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Ultrastructural investigations of dormant and germinated sporangiospores ofRhizopus arrhizus were conducted by the use of thin sectioning and freeze-etching procedures. Dormant spores contained deep furrows and prominent ridges with swellings along the sides of the ridges. The furrows and ridges almost disappeared as spores swelled during germination. The plasma membranes contained protuberances or depressions (approximately 50 nm diam) depending upon the nature of the fracture. Mitochondria in dormant spores were spherical and contained few cristae compared to mitochondria of germinated spores which were larger, more diverse in shape and contained abundant cristae. Treatments of spores with 20–25% glycerol prior to freeze-etching or thin sectioning resulted in the production of artifacts between the cell wall and the plasma membrane. Vesicles were rarely observed at the apices of young germ tubes, but were present in abundance in growing hyphal tips. Surface views of young germ tubes revealed the presence of microfibrils.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01287290
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  • 5
    Electronic Resource
    Electronic Resource
    Measurements of the criticalPO2 of renal mitochondria (1971)
    Springer
    Pflügers Archiv 323 (1971), S. 250-257 
    Details
    ISSN: 1432-2013
    Keywords: Renal Mitochondria ; O2-Consumption ; CriticalPO2 ; Flow-Limitation of O2-Consumption ; Nierenmitochondrien ; Sauerstoffverbrauch ; kritischer Sauerstoffdruck ; Flußlimitierung der Sauerstoffaufnahme
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Der kritische Sauerstoffdruck von isolierten Mitochondrien aus der Rinde und der äußeren Medulla der Rattenniere wurde mit der O2-Platinelektrode polarographisch gemessen. Mit Succinat als Substrat ergaben sich mittlere kritische mitochondriale Sauerstoffdrucke von 1,54 Torr (SD±0,58) bei 37°C und 1,01 Torr (SD±0,45) bei 25°C. Mit Malat als Substrat betrugen die mittleren kritischen Sauerstoffdrucke 0,92 Torr (SD±0,28) bei 37°C und 0,65 Torr (SD±0,25) bei 25°C. Dabei fand sich ein positiver linearer Zusammenhang zwischen der Höhe des Sauerstoffverbrauchs und der des kritischenPO2. Die Ergebnisse werden mit Daten über den Sauerstoffverbrauch der Ratten- und Hundeniere in situ und mit Sauerstoffdrucken, die ebenfalls in der Nierenrinde der Ratte und des Hundes in situ gemessen wurden, verglichen. Auf der Basis der hier vorgelegten kritischen mitochondrialen Sauerstoffdrucke und der niedrigsten Sauerstoffdrucke, die im Nierenrindengewebe gemessen wurden, wird eine zusätzliche Erklärung für die Flußlimitierung der Sauerstoffaufnahme der Säugetierniere entwickelt.
    Notes: Summary The criticalPO2 of isolated mitochondria from the cortex and outer medullary region of the rat kidney was polarographically measured using the O2-platinum-electrode. With succinate for substrate at 37°C and 25°C mean criticalPO2-values of 1.54 Torr (SD±0.58) and 1.01 Torr (SD±0.46) were found resp. Using malate for substrate the corresponding mean values were 0.92 Torr (SD±0.28 and 0.65 Torr (SD±0.25). A positive linear relationship between O2-uptake and criticalPO2 was observed. The results are compared with data on the O2-consumption of rat and dog renal cortex in situ and withPO2-values measured in the same organs. On the basis of the results here presented with regard to the lowestPO2-values found in the renal cortex of the dog an additional explanation of the flow limitation of the renal O2-consumption is developed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00586387
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  • 6
    Electronic Resource
    Electronic Resource
    Physiological studies on Phymatotrichum omnivorum VI. Lipid composition of mycelium and sclerotia (1974)
    Springer
    Mycopathologia 52 (1974), S. 261-266 
    Details
    ISSN: 1573-0832
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The lipid composition of the mycelium and sclerotia ofPhymatotrichum omnivorum was compared. The lipids of the mycelium contained 47.9 % polar lipids as compared to 21.4 % in the sclerotia. Sterols represented 10 % of the lipids in sclerotia as contrasted to 3.6 % of the mycelium. More monoglycerides (17.5 %) were detected in the sclerotia as compared to the mycelium (1.6 %). Fatty acid analysis indicated that linoleic acid was the predominant fatty acid in the total fatty acids fraction in both the mycelium and the sclerotia. Palmitic acid was the major free fatty acid in the mycelium, whereas myristic acid was the predominant free fatty acid in the sclerotia. In the fatty acids of the diglycerides of sclerotia, palmitic acid represented 71 % of that fraction, as compared to 6.6 % of the fatty acids of the diglycerides in the mycelium. The major fatty acid in the diglycerides of the mycelium was oleic acid.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02198752
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  • 7
    Electronic Resource
    Electronic Resource
    The physiological effects of fluorocarbon liquids in blood oxygenation (1970)
    Hoboken, NJ : Wiley-Blackwell
    Journal of Biomedical Materials Research 4 (1970), S. 313-339 
    Details
    ISSN: 0021-9304
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: The objective of this study was to determine the feasibility of using a fluorocarbon liquid in a liquid-liquid contacting device for extracorporeal blood oxygenation. Extraction experiments were carried out wherein blood was gently contacted with FC-43 (a fluorinated tributyl amine) for up to 48 hr. The fluorocarbon caused no significant change in red cell volume, white cell count, plasma hemoglobin, platelet count, plasma fibrinogen, or the relative amounts of thirteen different plasma proteins. To determine toxicity, FC-43 slugs and emulsions were injected intravenously and intraaortically into dogs. No chemical toxicity has been found, but the fluorocarbon is intolerable above about 1.7 mg/kg because it retards the flow of blood through pulmonary capillaries. FC-43 was used for several in vivo tests of a vertical cylindrical blood oxygenator. The hemolysis caused by the falling film of fluorocarbon on the inner Teflon wall of the cylinder was considerably less than that caused by direct contact between blood and Teflon, suggesting that the blood-fluorocarbon interface is quite atraumatic. All these results indicate that this fluorocarbon liquid holds considerable promise for use in a liquid-liquid blood oxygenator.
    Additional Material: 14 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jbm.820040305
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