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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 17 (1983), S. 0 
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Human lymphocytes alloactivated in vitro were cloned by limiting dilution in the presence of filler cells and interleukin 2 (IL 2)-containing supernatants of phytohaemagglutinin-stimulated lymphocytes. Clones with allospecific proliferative reactivity (PLT clones), measured by tritiated thymidine (3H-TdR) incorporation, were selected for extensive IL 2-dependent expansion. The cloned lines had finite lifespans, ranging from an estimated minimum of 28 to 〉 65 doublings. Function as PLT reagents, however, was retained in all cases for only an estimated 30 cell doublings. This apparent cessation of function was not caused by loss of the ability to metabolize thymidine, since lines continuing to grow for 〉 30 doublings still incorporated 3H-TdR in the presence of IL 2. An altered requirement for stimulating antigen (number of stimulating cells), or altered response kinetics, did not contribute to loss of PLT function. Exogenous IL 2 added during restimulation to responders previously ‘rested’ overnight without IL 2 did not restore the response. Thus, under present experimental conditions, functional lifespans of cloned PLT reagents appear fixed at ˜ 30 cell doublings.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Clinical and experimental pharmacology and physiology 9 (1982), S. 0 
    ISSN: 1440-1681
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: 1. The effect on renal function in sheep of inhibiting converting enzyme with captopril was examined before and after 5 days ACTH administration.2. Glomerular filtration rate, effective renal plasma flow, effective renal blood flow, mean arterial pressure and plasma sodium were all significantly incereased by ACTH treatment and plasma potassium was decreased. Captopril (20 mg i.v.) had no effect on renal function or blood pressure before or after ACTH treatment, although urinary potassium excretion decreased following captopril on day 6 of ACTH treatment.3. The increase in glomerular filtration rate and effective renal plasma flow seen with ACTH treatment in sheep does not appear to be mediated by the renin-angiotensin system.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-2048
    Keywords: Concanavalin receptors ; Endoplasmic reticulum ; Lepidium ; Statocytes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Using Concanavalin A (Con A) labeled with fluorescein isothiocyanate, we studied the intracellular localization of receptor molecules in the calyptra of 24-h dark-grown cress roots. Fixation in glutaraldehyde gave positive binding of the distal complex of the endoplasmic reticulum and the nucelus in the statocytes. In contrast, fixation in formaldehyde did not preserve the membrane-associated receptors, but revealed Con A affinity of the starch grain surface within the amyloplasts. Treatment of glutaraldehydefixed sections with non-ionic detergents led to partial solubilization of membrane components: the starch grain surface turned positive, though the positive binding of Con A to the endoplasmic reticulum and the nucleus remained unaffected. We therefore conclude that the Con A receptor in the membrane is a glycoprotein tightly inserted in other components of the compartment.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-2048
    Keywords: Amyloplasts ; Enzyme conversion ; Phosphorylose (starch) ; Senescence ; Solanum ; Starch phosphorylase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Phosphorylase was purified from young and senescent potato tubers. Antibodies raised against the enzyme from young tubers crossreacted with phosphorylase from old tissue, although the latter exhibited different physico-chemical properties. In polyacrylamide gel electrophoresis it migrated with higher mobility, its subunit molecular weight was determined in the range of 40,000 in contrast to 100,000 of the phosphorylase in young tubers. The enzyme of senescent tubers displayed an isoelectric point of 5.4 different from the one of young tubers with 5.0, and the diffusion coefficients of the two enzymes varied. The appearance of the phosphorylase form typical for senescent tissue is connected with changes in the intracellular localization as revealed by immunofluorescence. Before massive starch accumulation is initiated, non-vacuolated subepidermal cells contain antigenically active material in their cytoplasm. During starch accumulation in fully differentiated storage parenchyma, only amyloplasts fluoresce, indicating the presence of adsorbed phosphorylase protein. Cytoplasmic phosphorylase can be detected in the continuance of senescence and, finally, after 16 months of tuber storage, the particle-bound enzyme had mostly disappeared. Simultaneously, we observed membrane destruction and decomposition on the ultrastructural level. The phosphorylase from senescent potatoes is a converted molecule and seems to be formed by proteolytic cleavage. The location of phosphorylase in the amyloplasts during starch synthesis indicates that it also plays a role in starch synthesis and not only in its degradation.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Archives of gynecology and obstetrics 205 (1968), S. 151-161 
    ISSN: 1432-0711
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 211 (1966), S. 611-612 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Fig. 1. Sediment transport in the western North Atlantic (ref. 6). Physiographic diagram (ref. 7) In November 1965, on a cruise of the Duke University R.V. Eastward, a series of bottom photographs were taken between Cape Hatteras and Bermuda (Table 1 and Fig. 1). One of the stations on the ...
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Probability theory and related fields 58 (1981), S. 125-138 
    ISSN: 1432-2064
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    European journal of wildlife research 26 (1980), S. 126-132 
    ISSN: 1439-0574
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Summary In a wild boar enclosure in Austria, one individual several years old was observed smell marking and defending food. Apples were smell marked with urine and saliva. Young animals did not eat this food after having tested it by sniffing, although the marking ♂ did. The wild boar defended his feeding ground against men. In the process, he showed in a weaker or ritualised form behavioural patterns from the innate characteristics of parading, threatening and fighting. In addition, there is a report of an old boar in which acoustic impulses (the noises of a food container being used by another boar) triggered off a demonstration of rank and appropriation at the feeding ground. This trigger was more forceful than „avoidance of the enemy“. Mention is made of a case of imitation and learning in the overcoming of an obstacle.
    Abstract: Résumé Dans un parc à sangliers situé en Autriche un sanglier mâle adulte a été observé lorsqu'il procédait à des marquages olfactifs et lorsqu'il défendait sa nourriture. Des pommes furent marquées olfactivement au moyen d'urine et de salive. Contrairement au mâle en question, des sujets juvéniles n'ont pas absorbé cette nourriture apreast en avoir goûté par voie olfactive. Le verrat défendit son lieu de nourrissage contre les humains. A cette occasion il manifesta, sous une forme atténuée ou ritualisée, un comportement spécifique de défi, de menace et de combat. On fait également état d'une démonstration de rang social et d'appropriation sur une aire de nourrissage par un sanglier mâle âgé déclenchée par des stimuli acoustiques simulant le bruit provoqué par l'utilisation de mangeoires par d'autres sangliers. Ce déclencheur était plus puissant que l'„évitement d'un ennemi“. On signale un cas d'imitation et d'apprentissage lors du franchissement d'un obstacle.
    Notes: Zusammenfassung In einem österreichischen Wildschweingatter wurde ein mehrjähriger Keiler beim Duftmarkieren und Verteidigen von Futter beobachtet. Mit Harn und Speichel wurden Äpfel geruchlich markiert. Jungtiere haben dieses Futter nicht verzehrt, nachdem sie es olfaktorisch geprüft hatten, wohl aber das markierende ♂. Gegenüber Menschen verteidigte der Keiler seinen Futterplatz. Dabei zeigte er in abgeschwächter od. ritualisierter Form Verhaltensweisen aus dem innerartlichen Imponier-, Droh- und Kampfverhalten. Es wird ferner berichtet über einen alten Keiler, bei dem akustische Reize, die Geräusche eines von anderen Wildschweinen benutzten Futterbehälters, eine Rang- und Inbesitznahmedemonstration am Futterplatz auslösten. Dieser Auslöser war stärker als die „Feindvermeidung“. Ein Fall von Nachahmung und Lernen bei der Überwindung eines Hindernisses wird erwähnt.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    European food research and technology 138 (1968), S. 307-307 
    ISSN: 1438-2385
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Human genetics 〈Berlin〉 65 (1984), S. 242-247 
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The Dwl specificity was highly correlated with the serologically determined HLA-DR1 antigen in the Eighth International Histocompatibility Workshop 1980. By testing a large number of HLA-Dwl, DR1 defined homozygous typing cells (HTC) in a checkerboard primary mixed lymphocyte reaction, on a panel of about 30 HLA-DR1 heterozygous individuals, and in family segregation, three Dwl “subtypes” could be defined in association with certain HLA-A, -B, and -C-antigens. HTC TA, FRI, and FRA carrying the HLA haplotypes A11, B35, Cw4 or A3, B35, Cw4 in the homozygous state gave positive typing results with most HLA-DR1 positive panel members and stimulated only four other Dw1 HTCs (SRR≦35%). In contrast to this operationally “broad” specificity, Dw1-HTC-HEN (HLA-A2, B44, C-, homozygous) was non-stimulatory to all HTCs except one, but gave high responses against these, leading to the definition of a “narrow” specificity included in the “broad” one. Another such “narrow” specificity was represented by HTC FEE (HLA-A2, B27, Cw2 homozygous). Typing patterns with FEE were mostly different from those defined with other HTC. In family studies a specific typing pattern for this HTC could be shown to segregate with HLA. However, within some of these responses a contribution of the HLA haplotype in the trans position must be assumed.
    Type of Medium: Electronic Resource
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