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  • 1
    Electronic Resource
    Electronic Resource
    Some histochemical observations on the effects of chorioallantoic grafts on the spleen, bursa, and peripheral blood of chicken embryos (1966)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 118 (1966), S. 149-165 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Hatching eggs from inbred lines of chickens (inbreeding coefficient exceeds 95%) which show various degrees of resistance and susceptibility to Rous sarcoma, were used for experimentation. Adult tissues were grafted onto the chorioallantois on the tenth day of incubation and tissues of host and control embryos were harvested on the twentieth day of incubation. Enzymes were localized in tissues by histochemical procedures.Small pieces of tissue (thymus or bursa), when grafted onto the chorioallantois, increased the size of the spleen in host embryos although splenomegaly did not invariably occur. Two types of reactions were observed in the spleen, i.e., enlarged spleens with cysts or enlarged spleens which from a morphological point of view were normal. Grafts of either thymus or bursa decreased the size of the host embryo's bursa or were without effect. When weight of the bursa of host embryos was significantly less than that of control embryos on the twentieth day of incubation, this size relationship persisted in chicks four weeks post hatching.Intensity of dehydrogenase and acid phosphatase reactions in cysts of enlarged spleens and in the multinucleated giant cells investing them suggests that they consist of groups of degenerating cells. Intensity of enzyme reaction indicates that enlarged spleens of host embryos in which cysts were absent were normal. Enzyme reactions in the bursae of experimental embryos were more intense than those identified in the same tissues of control embryos. Catabolic reactions were the predominant type in grafts ten days subsequent to implantation. Grafts increased the number of erythrocytes in the peripheral blood of host embryos.
    Additional Material: 4 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051180202
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  • 2
    Electronic Resource
    Electronic Resource
    Mode of action of straight chain hydrocarbons on primary chemoreceptors of the blowfly, Phormia regina (1966)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 67 (1966), S. 53-62 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The fundamental actions of straight-chain hydrocarbons on the three known primary chemoreceptor cell types of the blowfly, Phormia regina, were studied in quantitative terms. Lower alcohols and long-chain amines act in two stages: first a reversible inhibition, and then injury, of the salt, water, and sugar receptors in the labellar sensilla. The primary effects on salt and water receptors resemble hydrocarbon narcosis of nerve. Effects on sugar receptors, when analyzed kinetically, superficially resemble competitive inhibition. Other evidence, however, indicates that a non-specific effect on sugar receptor sites is more likely. The electrophysiological results from labellar chemoreceptors indicate that the previously reported hydrocarbon behavioral rejection thresholds are best explained by the inhibition of the sugar receptors. This conclusion is strengthened by further electrophysiological and behavioral tests on tarsal chemoreceptors.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040670107
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  • 3
    Electronic Resource
    Electronic Resource
    Histochemical and electron microscopic observations on the salt secreting lacrymal glands of marine turtles (1966)
    New York, NY [u.a.] : Wiley-Blackwell
    American Journal of Anatomy 118 (1966), S. 337-357 
    Details
    ISSN: 0002-9106
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The lobular, compound, branched, tubular, salt-secreting lacrymal glands of two marine turtles, Chelonia mydas and Caretta caretta are similar in structure and in histochemical reactivity. Blood from the centrolobular arteries flows through a rich capillary bed counter to the flow of tubule secretion. The capillary endothelium is reactive for adenosine triphosphatase (ATPase). Nerves containing cholinesterase pervade the connective tissue. At the blind ends of the secretory tubules small basophilic peripheral cells contain an abundance of glycogen, monoamine oxidase (MAO) and phosphorylase but little succinic dehydrogenase (SDH) or cytochrome oxidase (CTO). Non-mitochondrial ATPase is concentrated at the luminal interface of these cells. The larger principal cells, lining the major portion of the secretory tubules, are rich in SDH and CTO but contain relatively little glycogen, MAO or phosphorylase. Broad intercellular channels reactive for mucopolysaccharide are formed by intermeshing, pleomorphic microvilli that fringe the extensive lateral surfaces of the principal cells. The cytoplasm of these cells contains profiles of smoothsurfaced endoplasmic reticulum (SSER), abundant mitochondria, and prominent Golgi membranes. Profiles of SSER and small membrane bound vesicles fill the apical cytoplasm but mitochondria are lacking. The luminal secretory border of the cell is extremely limited in area.Two types of epithelial cells line the duct system: basal cells that react strongly for non-specific esterase and MAO; and goblet cells containing mucopolysaccharide, acid phosphatase, cholinesterase, and ATPase.The principal cells, close to the arterial blood supply, contain the highest concentrations of oxidative enzymes and have special modifications of the cell surface consistent with their role in salt concentration and secretion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aja.1001180203
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  • 4
    Electronic Resource
    Electronic Resource
    The fine structure of daphnid supraesophageal and optic ganglia, and its possible functional significanceThis research was supported in part by National Science Foundation grant GB-3960; the assistance of Electron Microscope Laboratory staff at Washington State University is gratefully acknowledged. (1968)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 126 (1968), S. 373-393 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Neurosecretory activity and fine structure of the supraesophageal and optic ganglia of Daphnia schødleri Sars were studied. The relative amount of paraldehyde fuchsin stainable material present was determined at “daylight” and at one and three hours following for animals maintained under photoperiods of 7.5, 10.5, 13.5, and 16.5 hours. More material was found after one hour in both ganglia and there was a tendency for more in the optic ganglion under 7.5- and 10.5-hour photoperiods.Sections made at two levels in the supraesophageal ganglion and one level in the optic ganglion were examined with an electron microscope. Posterior and anterior parts of the supraesophageal ganglion contain apparent nerve processes at the edge of the ganglion, parallel to the anterior-posterior axis; these have large granules. Neurons in both areas contain patches of presumed polysaccharide granules. In the posterior region are a dorsolateral and a ventrolateral glandular cell, presumably these occur on both sides of the brain. They have very well-developed endoplasmic reticulum and some large granules. The dorsal cell is usually at the tip of a glial attenuation.Concentric lamellar systems are located in dilated nerve processes of the first optic ganglion neuropile. Large whorls (about 3.5 μ) are composed of concentric lamellae. When lamellae do not form complete rings, they end in loops or in dilated tubules that are sometimes constricted as vesicles. Small whorls (1.5 μ) typically have lamellae joined into two or three thick layers. Mitochondria are frequently associated with the whorls. It is proposed that the whorls are active in synthesis, possibly neurohumor production.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051260402
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  • 5
    Electronic Resource
    Electronic Resource
    Some histochemical and biochemical observations on the lymphatic tissues of highly inbred white Leghorns (1968)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 125 (1968), S. 281-301 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Lymphatic tissues of inbred lines of White Leghorn chickens and 20-day embryos (inbreeding coefficient exceeds 95%) were used in the experiments, e.g. line 6 was susceptible, line 7 was resistant, and line 15 I was intermediate in response to the virus. Enzyme reactions were studied in cryostat-cut sections of tissues and in tissue minces by colorimetric procedures. Numbers of isozymes and proteins of lymphatic tissues were resolved by disc gel electrophoresis.Colorimetric tests showed that intensities of lactate, malate, isocitrate and succinic dehydrogenase catalyzed reactions were higher in the bursae of 15 I 20-day embryos than they were in bursae of either line 6 or 7 embryos. Intensity of dehydrogenase reactions of the spleen (15 I embryos) exceeded that found in line 6 and 7 embryos. Intensity of diaphorase reactions in the spleen and thymus was fairly uniform in all lines of embryos. Intensity of DPN diaphorase reactions in the bursae of line 15 I embryos exceeds that found in either line 6 or 7 embryos. Intensity of enzyme reactions leveled off to become fairly uniform in lymphatic tissues of chickens 3-4 weeks post hatching with the exception that dehydrogenase reactions were less intense in the thymus of 15 I chickens.Photodensitometer scans of acrylamide gel columns showed that proteins of line 6 lymphatic tissues combined with less Amido black 10B than lymphatic proteins of either line 15 I or 7 embryos. There was fairly good agreement between concentrations of strong mobility (components 1-9) and weak mobility (components 10-16) in lymphatic tissues of all lines of embryos with the exception that strong mobility proteins were about twice as concentrated in line 15 I bursae. Variable numbers of lactate isozymes were found in the lymphatic tissues of 20-day embryos.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051250303
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