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  • 1965-1969  (7)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    European food research and technology 129 (1966), S. 160-160 
    ISSN: 1438-2385
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Zusammenfassung Es werden Abänderungen der Milcheiweißbestimmung nachKutscher, Nagel undPfaff vorgeschlagen, mit deren Hilfe eine bessere Reproduzierbarkeit dieser Bestimmung erreicht werden kann.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Pflügers Archiv 285 (1965), S. 360-372 
    ISSN: 1432-2013
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 1. The concept of glomerular tubular balance implies the constancy of fractional reabsorption of glomerular filtrate by the proximal convoluted tubules despite alterations in the arterial blood pressure or GFR. This concept was investigated in the kidney of the nondiuretic rat during acute alterations in arterial pressures from 50–160 mm Hg. 2. Three different methods independent of each other were used instead of the conventional approach of measuring the inulin TF/P ratios alone: a) determination of the transporting capacity of proximal tubules by the split oil droplet method; b) estimation of the passage time of fluid through the proximal convoluted tubules an c) measurement of the inulin TF/P ratios. 3. Transporting capacity of the proximal tubule was constant and independent of the arterial pressure. Proximal passage time became shorter as arterial pressure was raised from 50–160 mm Hg. Increase in GFR was followed by shortening of proximal passage time. Inulin TF/P ratios in proximal convolutions increased with longer and decreased with shorter passage times. The fractional reabsorption decreases when the arterial pressure is raised and increases when it is lowered. These results do not support the concept of the existence of glomerular tubular balance in the nephrons of nondiuretic rats during acute changes in arterial pressure.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-2013
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The pressure in the glomerular capillaries of the rat kidney was determined by micropuncture of individual nephrons. The proximal tubule was blocked by injection of viscous oil. The intratubular hydrostatic pressure increased until it reached a steady state, the intratubular stop-flow pressure. Since the glomerular capillary wall is an ultrafiltration membrane, impermeable to proteins, the sum of intratubular stop-flow pressure plus the plasma colloid osmotic pressure should equal the pressure in the glomerular capillaries. This pressure increased as the arterial pressure was raised from 60 to 90 mm Hg. Thereafter it remained constant at 88±4 mm Hg despite further elevation of the arterial pressure up to 160 mm Hg. Determination of the stop-flow pressure in Bowman's capsule and in the proximal tubules of denervated kidneys gave the same results as above. The hydrostatic pressure drop in the afferent arterioles was determined by subtracting the glomerular capillary pressure from the arterial pressure. This pressure drop was negligible at arterial pressures below 90 mm Hg and increased linearly as the arterial pressure was elevated from 90 to 160 mm Hg. Both the above findings are compatible with and confirm the principle of autoregulation of renal blood flow and glomerular filtration rate.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Pflügers Archiv 291 (1966), S. 99-106 
    ISSN: 1432-2013
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Having demonstrated that the rat parotid gland is able to excrete a hypotonic saliva, we studied the formation of saliva in the different segments of the duct system, through micropuncture and microanalytical techniques. The duct system of the gland is composed of three functionally different segments, acini-intercalated ducts, striated ducts and excretory ducts. a) Acini-intercalated ducts secrete a primary fluid with plasma-like osmolality and sodium and potassium concentration. b) Striated ducts modify the primary fluid by active reabsorption of sodium in excess of water, thus producing hypotonicity. c) Excretory ducts serve as conduits of the saliva to the mouth. A process of reequilibration of the duct fluid to isotonicity occurs in the excretory ducts of the resting gland. Secretion of small amounts of potassium also appears to take place in these ducts.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-2013
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The fresh water animal Asplanchna priodonta has a pair of protonephridia which have been considered excretory organs by many investigators. We have studied the ultrastructure and function of the protonephridium of this animal by using electron microscopy, micropuncture, and ultramicroanalysis of minute samples of body fluid and urine. It was shown that this primitive excretory organ is a syncytium with complex organization. It has a part where filtration of body fluid takes place (terminal organs) and a part where reabsorption of water, sodium and other solutes of the filtered fluid occurs (protonephridial tubule). The body fluid of the animal is hypertonic to the surrounding medium (osmolarity of 81.0 vs. 17.7 mOsm/l). This is maintained by the excretion of urine hypotonic to and with less sodium than the body fluid. When the animal is placed in distilled water a mild dilution of the body fluid occurs initially. The protonephridium reacts by increasing the excretion of water and decreasing the excretion of sodium and other solutes in the urine. Thus, the homeostasis of the body fluid is maintained. This study shows that the primitive protonephridium functions on the same filtration—reabsorption of body fluid (or plasma) basis as the more developed nephridia of higher invertebrates and the nephrons of the vertebrate kidneys. In Asplanchna priodonta, the course of fluid during urine formation is from the body cavity—terminal organ—tubule—exterior. This is identical to the course of fluid from blood—coelomic cavity (or Bowman's capsule)—tubule—exterior found in the excretory organs of the higher invertebrates and vertebrates. It appears that such a course of fluid is basic for the function of excretory organs.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Pflügers Archiv 290 (1966), S. 184-192 
    ISSN: 1432-2013
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The rat parotid has been considered a salivary gland that produces saliva isotonic to plasma and with high sodium concentrations at all flow rates. We studied the excretion of sodium, potassium and total solute in the unstimulated and pilocarpine stimulated parotid of adult rats. The effects of retrograde injection of ouabain into the duct system of the gland were also investigated. Use of microanalytical methods enabled us to study the composition of the saliva at all flow rates. The saliva was isotonic to plasma at resting flow rates. Minimal increase in flow rate was associated with a precipitous drop in osmolarity to as low as 86 mOsm/l. Further increase in flow rate up to 135 mg/min/gram wet gland tissue, was followed by gradual increase of osmolarity towards isotonicity. The changes in osmolarity were mainly caused by changes in the sodium concentration. The potassium concentration of the saliva was 3–4 times that of plasma and showed little variation with changing flow rates. Retrograde injection of ouabain into the duct system of the gland up to the acini has no effect on the flow rate but partially inhibited the ability of the gland to elaborate hypotonic saliva. These findings demonstrate that, contrary to what has so far been accepted, the rat parotid is similar to the parotid of man and dog in the excretion of water and electrolytes. In addition, this work supports the hypothesis that the parotid saliva is formed by secretion of a plasma-like fluid in the acini-intercalated duct region, which is subsequently modified by active sodium reabsorption in excess of water in another site (or sites) of the duct system of the gland.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    European food research and technology 130 (1966), S. 273-276 
    ISSN: 1438-2385
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Zusammenfassung Die qualitative und quantitative Bestimmung von Gluconsäure-δ-lacton in schnittfesten Dauerwürsten ist über Gluconsäure möglich. Es wird eine dünnschichtchromatographische Vorprobe und eine quantitative Bestimmung beschrieben, die auf der Oxydation von Gluconsäure mit Perjodsäure beruht.
    Type of Medium: Electronic Resource
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