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  • 1965-1969  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Inactivation of a newly transferred gene in recombination-deficient recipients of Escherichia coli (1969)
    Springer
    Molecular genetics and genomics 103 (1969), S. 305-312 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The expression of a newly transferred lacZ + gene in lacZ recipients carrying various mutations in the recA and recB genes was studied by measuring the rates of induced synthesis of β-galactosidase in zygotes formed after mating with either F′ or Hfr donors. The ability to synthesize β-galactosidase decreases with time in both recA and recB zygotes when the lacZ + gene is transferred from an Hfr donor, but not when the lacZ gene is transferred from an F′ donor. There is no such inactivation of the newly transferred lacZ + gene in Rec+ zygotes. We conclude that the functioning of the transferred DNA is progressively inactivated in rec recipients unless the DNA is contained in an episome such as F′.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00383480
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Map location of arginyl-tRNA synthetase mutations in Escherichia coli K-12 (1969)
    Springer
    Molecular genetics and genomics 104 (1969), S. 383-390 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mutants of Escherichia coli K-12 previously isolated in the authors' laboratory have reduced arginyl-tRNA synthetase activity. The mutants fall into two classes. All mutants grow slowly on arginine-free medium. On arginine-supplemented medium some mutants grow at a normal rate (Class I) while others still grow slowly (Class II). Matings were performed to located a Class I and a Class II mutation on the E. coli chromosome map, and on the basis of our results we have assigned both to one locus, argS.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00334238
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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