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  • 1
    Digitale Medien
    Digitale Medien
    Monoclonal antibody-mediated cytotoxicity against rat Beta cells detected in vitro does not cause Beta-cell destruction in vivo (1992)
    Springer
    Diabetologia 35 (1992), S. 608-613 
    Details
    ISSN: 1432-0428
    Schlagwort(e): Monoclonal islet cell surface antibodies (mcICSA) ; anti-islet cell toxicity ; application in vivo ; pancreatic insulin content ; rat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Two monoclonal Beta-cell surface antibodies M10H6 und K14D10 were obtained by fusion of spleen cells of Balb/c mice with the myeloma cell line P30. The monoclonal antibody M10H6 was induced by immunization with rat insulinoma cells finally boostered with disintegrated rat islets, whereas the K14D10 was generated after immunization with porcine proinsulin. Both monoclonals belong to the IgG2A isotype and were screened with insulin-producing rat insulinoma cells by an indirect immunofluorescence test as well as by a cellular enzyme linked immunosorbent assay. In addition to the cell surface binding on living Beta cells the monoclonals react with islets on cryostat sections of rat pancreas. The anti-islet cytotoxic potential of these monoclonals was measured by 51Chromium-release in the presence of complement or Fc-receptor bearing leucocytes using 51Chromium-labelled rat islet cells as target. Both antibody secreting hybridomas were propagated in syngeneic mice resulting in high levels of islet cell surface antibodies in ascites and sera from the recipient. High anti-islet cytotoxicity was mediated by ascites fluid, but no mouse developed hyperglycaemia. Furthermore, the repeated injections of the monoclonals into rats did not exert a diabetogenic action and failed to reduce the pancreatic insulin content although the attraction of the K14D10 to the pancreatic islets in vivo could be demonstrated. We conclude that islet cell surface antibody-mediated Beta-cell lysis in vitro may not be relevant to Beta-cell destruction in vivo.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00400250
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  • 2
    Digitale Medien
    Digitale Medien
    Irrtum umSynchytrium (1964)
    Springer
    Theoretical and applied genetics 34 (1964), S. 229-229 
    Details
    ISSN: 1432-2242
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00705899
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  • 3
    Digitale Medien
    Digitale Medien
    Electrophoretic screening for human apolipoprotein C-II variants: repeated identification of apolipoprotein C-II(K19T) (1995)
    Springer
    Journal of molecular medicine 73 (1995), S. 373-378 
    Details
    ISSN: 1432-1440
    Schlagwort(e): Apolipoprotein C-II ; Hypertriglyceridemia ; Polymerase chain reaction ; Mutation ; Phenotypic variability
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Screening for apolipoprotein (apo) C-II variants in the plasma of 400 students, 600 patients of a cardiological rehabilitation center, and 1200 patients of an outpatient lipid clinic by isoelectric focusing and subsequent anti-apo C-II immunoblotting led to the identification of four individuals whose plasma samples contained an apo C-II isoform with an abnormal isoelectric point. In all cases direct sequencing of PCR-amplified DNA assessed a heterozygous A to C transversion in codon 19 of the apo C-II gene which leads to the replacement of lysine with threonine. Two of the four index patients presented with moderate hypertriglyceridemia; one suffered from severe hyperlipidemia, with triglyceride levels ranging between 180 and 1900 mg/dl, depending on dietary changes. Sequencing of this proband's lipoprotein lipase gene showed no alteration compared to the wildtype sequence. A study in his family revealed that heterozygosity for apo C-II(K19T) is not associated with differences in mean lipid and lipoprotein concentrations. In conclusion, apo C-II(K19T) occurs in Germany at a frequency of approximately 1 in 550. Although this variant is not sufficient to cause hypertriglyceridemia, it may be possible that apo C-II(K19T) causes hypertriglyceridemia in the presence of additional as yet unidentified environmental and/or genetic factors.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00192889
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  • 4
    Digitale Medien
    Digitale Medien
    Carbonsäureester von α-Dialkylamino-alkanolen (1960)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 72 (1960), S. 523-523 
    Details
    ISSN: 0044-8249
    Schlagwort(e): Chemistry ; General Chemistry
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/ange.19600721507
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