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Enzymatic Cycle of Inorganic Nitrogen in Animal Tissues (1960)

YAMAFUJI, K. ; OSAJIMA, Y. ; OMURA, H.

[s.l.] : Nature Publishing Group

Nature 185 (1960), S. 162-162

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ISSN: 1476-4687

Source: Nature Archives 1869 - 2009

Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics

Notes: [Auszug] An example of the procedure of preparing the enzyme solution was as follows: 30 gm. of hen liver were homogenized with 90 ml. of 0-1 M phosphate buffer of pH 7-5, and after 30 min. at 35 C., the homogenate was centrifuged at 3,000 r.p.m. for 10 min. at 0 C. The supernatant was treated with acetone ...

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1038/185162a0

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