ISSN:
1365-3083
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
Lymphocytes from 20 normal blood donors were stained with fluorescein isothiocyanate (FITC)-conjugated anti-F(ab′)2, anti-VHI, anti-VHII, and anti-VHIII subgroup antisera. The means of the percentages of staining were 2.9%, 5.0%, and 5.5% for the VHI, VHII, and VHIII subgroups, respectively. The sum of the percentages of the lymphocytes stained with each of the VH subgroup-specific antisera corresponded well with the percentages of lymphocytes stained with an anti-F(ab′)2 antiserum. In addition, it was shown by double immunofluorescence staining and in various depletion experiments and tests with thymocytes and lymphocytes from patients with hypogammaglobulinemia that the cells staining with anti-VH antisera corresponded to the membrane Ig-positive cells—that is, the B-lymphocyte population.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-3083.1976.tb00266.x
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