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Due to technical work, the interlibrary loan service wont be available from March 28th until presumably April 3rd.
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  • interleukin-2 gene  (2)
  • 1
    ISSN: 1573-8221
    Keywords: luliberin ; interleukin-2 gene ; mRNA synthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The ability of the antitumor analogs of luliberin (LH-RH), a hypothalamic peptide hormone, to stimulate the immune function of T cells is examined in experiments with the gene coding for interleukin-2 (IL-2). Recombinant MIL2C or 4×Pu DNA containing the marker gene of chloramphenicol acetyltransferase (CAT) under the control of a 2.2 kb promoter of murine IL-2 gene or four copies of purine-rich element (from −292 to −246 base pairs), respectively, is injected inXenopus laevis oocytes. The promoter activity is blocked by inoculation of the protein fraction of nuclear extracts from resting mouse splenic T cells. The IL-2 gene promoter is dereppressed after injection of the short LH-RH analog L1 (7 amino acid residues) into the oocyte nucleus or cytoplasm. The addition of L1 or L2 (an LH-RH analog consisting of 10 amino acid residues) to the incubation medium activates mouse splenic T cells and stimulates the synthesis of IL-2 mRNA 2- to 3-fold more intensely than ConA+rIL-2, judging from dot-blot andin situ hybridization data. Cytological analysis of cell culture shows that the presence of L1 and L2 peptides in the culture medium promotes differentiation of T cells. It is hypothesized that the antitumor activity of these peptides is associated with the stimulation of IL-2 synthesis.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-8221
    Keywords: interleukin-2 gene ; protein factors ; regulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The nuclear protein factors which stimulate synthesis of mRNA of interleukin-2 (IL-2) in the system of surviving murine T lymphocytes are isolated from cells of the spleen and brain of immunized rats. The active fractions of spleen and brain (13–19 and 25 kD, respectively), which interact with the promoter-enhancer sequences of the IL-2 gene localized in the region from +39 to −141 base pairs, are isolated by the method of high performance liquid chromatography from the total nuclear extract. The induction of luciferase synthesis is shown for protein factor-treated Jurkat cells transformed with p-IL-2LUC recombinant plasmid.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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