ISSN:
1434-0879
Keywords:
Prostate cancer
;
Tumor marker
;
Prostate-specific antigen
;
γ-Seminoprotein
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Prostate-specific antigen (PA) and γ-seminoprotein (γ-Sm) were compared by immunocytochemical, immunodiffusion and immunoblotting methods using rabbit anti-PA antibody and rabbit anti-γ-Sm antibody. Enzyme immunoassys (EIAs) were developed for measurements of PA and γ-Sm to determine a correlation between serum PA and γ-Sm levels in patients with prostate cancer. The patterns of localization and distribution of PA and γ-Sm were identical in prostate tissue sections, including benign and cancerous human prostacs. The immunodiffusion study showed that the antigens with which anti-PA antibody and anti-γ-Sm antibody reacted in seminal plasma and prostate tissue homogenates were identical to each other. In the immunoblotting study, anti-PA antibody and anti-γ-Sm antibody recognized a single antigen corresponding to a molecular weight of approximately 33,000 both in seminal plasma and prostate tissue homogenates. The EIAs developed in this study were sensitive, specific, and reproducible, and the correlation between serum PA and γ-Sm values determined by these EIAs was highly significant (r=0.99, P(0.001). These results indicated that PA and γ-Sm were immunologically identical and that serum PA and γ-Sm determined by immunoassays using anti-PA antibody and anti-γ-Sm antibody should be evaluated as identical tumor markers for serodiagnosis of prostate cancer.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00294017
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