ISSN:
0935-6304
Keywords:
Capillary zone electrophoresis
;
“Isotachophoretic effect”
;
Sample stacking
;
Absorbance jumps
;
pH gradient
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
During some capillary zone electrophoresis (CZE) experiments, the baseline UV absorbance signal at 200 nm “jumped” from one stable level prior to the water plug (marking the flow of neutrals) to another stable level after the water plug. The phenomenon was further examined with distilled water as the sample and with different buffers, applied potentials, and salt concentrations in the buffer. It seems that there is an “isotachophoretic effect” on top of the CZE separations when running under stacking conditions. The effect results in a higher pH value of the buffer after the water plug compared to the pH prior to the plug. The nature of the buffer, the salt concentration in the buffer, and the applied potential all affect this phenomenon.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jhrc.1240130911
Permalink
