ISSN:
1432-203X
Keywords:
Key wordsCucumis melo
;
1-Aminocyclopropane-1-carboxylate oxidase
;
Ethylene
;
Antisense RNA
;
Transgenic plant
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The role of ethylene in shoot regeneration was investigated using transgenic Cucumis melo plants expressing an antisense 1-aminocyclopropane-1-carboxylate (ACC) oxidase gene. ACC oxidase catalyses the last step of ethylene biosynthesis. Leaf and cotyledon explants from the transgenic plants exhibited low ACC oxidase activity and ethylene production, whereas the regeneration capacity of the tissues was greatly enhanced (3.5- and 2.8-fold, respectively) compared to untransformed control tissues. Addition of ethylene released by 50 or 100 µm 2-chloroethylphosphonic acid dramatically reduced the shoot regeneration rate of the transgenic tissues. The results clearly demonstrate that ethylene plays an important role in C. melo morphogenesis in vitro.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990050447
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