ZIB

Digitale Medien

A homopurine:homopyrimidine sequence derived from the rat neuronal cell adhesion molecule-encoding gene alters expression in transient transfections

Chen, A. ; Reyes, A. ; Akeson, R.

Amsterdam : Elsevier

Gene 128 (1993), S. 211-218

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ISSN: 0378-1119

Schlagwort(e): DNA-binding protein ; Recombinant DNA ; firefly luciferase ; gel retardation ; transcription regulation

Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Thema: Biologie

Materialart: Digitale Medien

URL: http://linkinghub.elsevier.com/retrieve/pii/0378-1119(93)90565-K

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Digitale Medien

Potentiometric method for subatrate analysis using immobilized NAD+-dependent oxidoreductase enzymes (1979)

Chen, A. K. ; Liu, C. C. ; Schiller, J. G.

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 21 (1979), S. 1905-1915

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ISSN: 0006-3592

Schlagwort(e): Chemistry ; Biochemistry and Biotechnology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Notizen: Two coenzyme-dependent oxidoreductases, glucose dehydrogenase and alcohol dehydrogenase, were immobilized in polyacrylamide gel over a platinum grid matrix and used as enzyme electrodes to measure their substrate concentrations in buffered aqueous solutions. The immobilized enzymes were used to oxidize their substrates in the presence of NAD+. Ferricyanide was used as the redox mediator and electroactive specific. The determinations of glucose and ethenol were utilized to demonstrate and evaluate the performance of the system. The described methodology should be readily applicable to the analysis of numerous other substrates of coenzyme-dependent oxidoreductases.

Zusätzliches Material: 6 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/bit.260211103

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Digitale Medien

Potentiometric quantitation of glycerol using immobilized glycerol dehydrogenase (1982)

Chen, A. K. ; Starzmann, J. A. ; Liu, C. C.

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 24 (1982), S. 971-975

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ISSN: 0006-3592

Schlagwort(e): Chemistry ; Biochemistry and Biotechnology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Notizen: Glycerol dehydrogenase was immobilized in polyacrylamide gel layered over a small platinum screen and used to catalyze the oxidation of glycerol. In the presence of NAD+ and potassium ferricyanide, the coupling reaction generated a measurable electrical potential which was found to be Nernstian with respect to the glycerol concentration range of 10-4M to 10-1M. The reproducibility of the measurement and the optimal conditions for glycerol determination were described.

Zusätzliches Material: 1 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/bit.260240416

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Digitale Medien

Electrochemical evaluation of lactate dehydrogenase immobilized in polyacrylamide gels (1977)

Chen, A. K. ; Liu, C. C.

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 19 (1977), S. 1785-1792

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ISSN: 0006-3592

Schlagwort(e): Chemistry ; Biochemistry and Biotechnology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Notizen: Lactate dehydrogenase (EC 1.1.1.27) has been immobilized in polyacrylamide gels over a platinum grid matrix. The immobilized enzyme is used to oxidize L-lactate in the presence of nicotinamide adenine dinucleotide (NAD+) and femcyanide. The NADH produced is then chemically oxidized back to NAD+by ferricyanide. The coupled reduction of ferricyanide ions to ferrocyanide ions results in a measurable electrochemical potential. This measurable zero-current potential is found to be Nernstian in nature and directly proportional to the logarithm values of L-Iactate concentration over the range of 2 × 10-5to 5 × 10-2M. The results indicate that immobilized lactate dehydrogenase can be incorporated into a system to detect L-Lactate acid in aqueous solutions.

Zusätzliches Material: 3 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/bit.260191205

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Digitale Medien

Solvent dependence of 13C relaxation times and reorientational dynamics in tribromobenzene (1988)

Chen, A. F. T. ; Wang, S. P. ; Schwartz, M.

Chichester : Wiley-Blackwell

Organic Magnetic Resonance 26 (1988), S. 675-678

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ISSN: 0749-1581

Schlagwort(e): Tribromobenzene ; 13C spin-lattice relaxation times ; correlation times ; Chemistry ; Analytical Chemistry and Spectroscopy

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Chemie und Pharmazie

Notizen: The 13C spin-lattice relaxation times and nuclear Overhauser enhancements of protonated carbons in 1, 3, 5-tribromobenzene were measured in a number of solvents of widely varying viscosity, Derived reorientational correlation times were found to increase approximately linearly with solution viscosity, as predicted by hydrodynamic theories of rotational diffusion.Rotational correlation times calculated from the perrin stick model were two to three times longer than the measured τc values. Similarly, correlation times predicted by the Hu-Zwanzig slip model were too small by a factor of two. On the other hand, application of the newer Hynes-Kapral-Weinberg theory furnished reorientational correlation times that were in virtually quantitative agreement with the experimental results.

Zusätzliches Material: 1 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/mrc.1260260807

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