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  • Restriction fragment length polymorphism (RFLP)  (2)
  • 14∶OAc  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 92 (1996), S. 702-708 
    ISSN: 1432-2242
    Keywords: Oryza ; Tandemly repeated DNA sequence ; Restriction fragment length polymorphism (RFLP)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A tandemly repeated DNA sequence (RRS7) was isolated from Oryza alta (CCDD). RRS7-related sequences were also found tandemly arrayed in genomes AA, BB, BBCC, CC, and EE, and a small amount of RRS7-related sequences were detected in genome FF and the Oryza species with unknown genomes. DNA sequence analysis of the 1844-bp insert of RRS7 revealed that it contained six tandemly repeated units, of which five were 155 bp in length and one was 194 bp in length and contained an imperfect internal 39-bp duplication. Southern blot analysis showed that the boundary sequence contained in RRS7 is a single-copy sequence. A 155-bp consensus sequence derived from the six monomeric repeats contained no internal repeat and showed no significant homology to other currently known sequences. The results of Southern blot and sequence analysis revealed that there are at least two subfamilies present in the RRS7 family; these are represented by the DraI site and the MspI site, respectively. Restriction digestion with two pairs of isoschizomers MboI/Sau3A and MspI/HpaII demonstrated that most of the C residues in the GATC sites and the internal C in the CCGG sites of the RRS7 family in O. Alta were methylated. The usefulness of the RRS7 family in determining the evolutionary relationship of the genome DD and other Oryza genomes is discussed.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 92 (1996), S. 702-708 
    ISSN: 1432-2242
    Keywords: Key words Oryza ; Tandemly repeated DNA sequence ; Restriction fragment length polymorphism (RFLP)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A tandemly repeated DNA sequence (RRS7) was isolated from Oryza alta (CCDD). RRS7-related sequences were also found tandemly arrayed in genomes AA, BB, BBCC, CC, and EE, and a small amount of RRS7-related sequences were detected in genome FF and the Oryza species with unknown genomes. DNA sequence analysis of the 1844-bp insert of RRS7 revealed that it contained six tandemly repeated units, of which five were 155 bp in length and one was 194 bp in length and contained an imperfect internal 39-bp duplication. Southern blot analysis showed that the boundary sequence contained in RRS7 is a single-copy sequence. A 155-bp consensus sequence derived from the six monomeric repeats contained no internal repeat and showed no significant homology to other currently known sequences. The results of Southern blot and sequence analysis revealed that there are at least two subfamilies present in the RRS7 family; these are represented by the DraI site and the MspI site, respectively. Restriction digestion with two pairs of isoschizomers MboI/Sau3A and MspI/HpaII demonstrated that most of the C residues in the GATC sites and the internal C in the CCGG sites of the RRS7 family in O. alta were methylated. The usefulness of the RRS7 family in determining the evolutionary relationship of the genome DD and other Oryza genomes is discussed.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 3
    ISSN: 1573-1561
    Keywords: Sex pheromone ; Ostrinia furnacalis ; E12–14∶OAc ; Z12–14∶OAc ; 14∶OAc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract (E)-12-Tetradecenyl acetate (E12–14∶OAc), (Z)-12-tetradecenyl acetate (Z12–143nOAc), and tetradecanyl acetate (14∶OAc) were extracted and identified as major chemical components from female tips of the Asian corn borer,Ostrinia furnacalis (Hübner) (Lepidoptera: Pyralidae) in Taiwan, with a combined wash ratio of 48∶37∶15 and an individual female ratio of 45∶39∶16. The average amount ofE12-,Z12–14∶OAc and 14∶OAc in each female gland was 6.6±4.6, 5.8±3.5, and 2.4±1.7 ng/female, respectively. The mixture of these three synthetic chemicals not only gave strong activities in male antennae but also could catch significantly more males than virgin females in field-trapping tests. The field test also showed no significant difference in trapping ability among the three-component Taiwan formulations, the two-component Taiwan formulation (Z12–14∶OAc andZ1214∶OAc, 53∶47), and the Japan formulation (Z12–14∶ OAc andE 12–14∶OAc, 3:2).
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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