ISSN:
1435-1463
Keywords:
Keywords: 1(N)-Amino-4-phenyl-1
;
2
;
3
;
6-tetrahydropyridine
;
oxidation
;
monoamine oxidase
;
MPTP
;
MPP+
;
human brain synaptosomes.
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary. A 1-amino analog of MPTP, 1(N)-amino-4-phenyl-1,2,3,6-tetrahydropyridine, was synthesized and the oxidation was examined using human synaptosomal mitochondria as sources of type A and B monoamine oxidase. An oxidation product, 1-amino-4-phenylpyridinium ion, was quantified by high-performance liquid chromatography-fluorometric detection. The amino analog was a substrate of both type A and B monoamine oxidase and the oxidation depended linearly on the enzyme amount and the reaction time with an optimal pH around 7.5. After the systemic injection of the amino analog in C57/black mice for one week, 1-amino-4-phenylpyridinium ion was detected in the brain. 1(N)-Amino-4-phenyl-1,2,3,6-tetrahydropyridine was proved to be cytotoxic to pheochromocytoma PC12 cells, and it may be a new neurotoxin bioactivated through the oxidation by type A and B monoamine oxidase.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s007020050128
Permalink
