ISSN:
1434-0879
Keywords:
Key words Vitamin C
;
N-acetyltransferase
;
2-aminofluorene
;
DNA adduct
;
Bladder cancer
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Previous studies have already demonstrated the protective role of vitamin C (ascorbic acid) in certain types of cancer. This study reports on the effects of vitamin C on arylamineN-acetyltransferase (NAT) activity and DNA adduct formation in a human bladder tumor cell (T24) line. The activity of NAT was measured using high-performance liquid chromatography (HPLC), by assaying for the amounts of acetylated 2-aminofluorene (AF) and p-aminobenzoic acid (PABA) and the remaining amounts of AF and PABA. T24 cells were used for examining NAT activity and carcinogen–DNA adduct formation. The results demonstrated that NAT activity and 2-aminofluorene–DNA adduct formation in T24 cells were inhibited and decreased by vitamin C in a dose-dependent manner. The apparent kinetic parameters (apparent values ofK m and V max) from T24 cells were also determined with and without vitamin C cotreatment. The data also indicated that vitamin C decreased the apparent values of K m and V max from T24 cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002400000130
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