ISSN:
1432-1912
Keywords:
3,4-Dihydroxyphenylacetic acid
;
3,4-Dihydroxymandelic acid
;
3,4-Dihydroxyphenylethanol
;
3,4-Dihydroxyphenylglycol
;
Radioenzymatic determination
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary An assay is described for the simultaneous determination of dopamine, noradrenaline, adrenaline, dopa, 3,4-dihydroxyphenylacetic acid, 3,4-dihydroxyphenyl-ethanol, 3,4-dihydroxymandelic acid and 3,4-dihydroxy-phenylglycol, capable of detecting amounts in the femtomol range. The assay is based on the O-methylation of the catechol moiety utilizing S-[3H-methyl]-adenosyl-l-methionine and a partially purified catechol-O-methyl transferase to form the various O-[3H-methyl]-catechol derivatives. The O-[3H-methyl]-catechol derivatives are purified by thin layer chromatography, solvent partitions and/or ion exchange chromatography. The assay was successfully applied to biological sample. It was possible for the first time, to detect free 3,4-dihydroxy-phenylglycol, free 3,4-dihydroxyphenylethanol and 3,4-dihydroxymandelic acid in a small volume (25μl) of blood plasma of man, rat, dog and rabbit. The conjoint measurement of catecholamines and their catechol metabolites in minute amounts of biological samples may contribute to a more detailed understanding of catecholamine metabolism in the peripheral and central nervous system.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00503307
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