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  • 1995-1999  (2)
  • 07.03.2 Comet C/1995 O1:Hale–Bopp  (1)
  • 3-D cell culture  (1)
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  • 1995-1999  (2)
Year
  • 1
    ISSN: 1573-0794
    Keywords: 07.03.2 Comet C/1995 O1:Hale–Bopp ; 13.18.4
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract The concept of simultaneous multifrequency continuum observations, successfully tested on Comet Hyakutake, was applied to Comet Hale-Bopp, using the Heinrich Hertz Submillimeter Telescope (HHT) with the four color bolometer between 250 and 870 GHz, the IRAM 30m telescope at 240 Ghz, the MPIfR 100-m telescope at 32 GHz, and the IRAM interferometer near 90 and 240 GHz. Near-simultaneous measurements were done between February 15 and April 26, 1997, mainly concentrated in mid March shortly before perigee of the comet. The measurements gave the following preliminary results: Interferometer detection of the nuclear thermal emission. If the signal at the longest interferometer spacing of 170 mis due to thermal emission from the nucleus only, its equivalent diameter is ∼49 km. If, however, this signal contains a contribution from a strongly centrally peaked halo distribution(e.g., r−2 density variation) the diameter may be as low as 35 km. The emission found interferometrically was always 5″ north and 0.1 sec east from the position predicted by Yeoman's solution 55.The comparison of the interferometric continuum emission with the simultanously obtained molecular line observations (reported on this conference) shows the origin of the strongest line emission concentrated on the nucleus. The 30-m observations show a radio halo with a gaussian FWHP of ∼11, corresponding to a diameter of 11000 km at geocentric distance of 1.2 a.u. A spectral index of ∼3.0 for the total signal, which may indicate a smaller mean particle size than for Hyakutake. Assuming an average cometary density of 0.5 gcm−3, the mass contained in the nucleus is ∼1$#x2013;3 1019 g and 1012 g in the particle halo.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 0197-8462
    Keywords: electromagnetic field ; myositis ossificans ; osteoblastic cells ; in vitro ; 3-D cell culture ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Human osteoblastic cells were grown in a three-dimensional (3-D) cell culture model and used to test the effects of a 20 Hz sinusoidal electromagnetic field (EMF; 6 mT and 113 mV/cm max) on collagen type I mRNA expression and extracellular matrix formation in comparison with the effects of growth factors. The cells were isolated from trabecular bone of a healthy individual (HO-197) and from a patient presenting with myositis ossificans (MO-192) and grown in a collagenous sponge-like substrate. Maximal enhancement of collagen type I expression after EMF treatment was 3.7-fold in HO-197 cells and 5.4-fold in MO-192 cells. Similar enhancement was found after transforming growth factor-β (TGF-β) and insulin-like growth factor-I (IGF-I) treatment. Combined treatment of the cells with EMF and the two growth factors TGF-β and IGF-I did not act synergistically. MO-192 cells produced an osteoblast-characteristic extracellular matrix containing collagen type I, alkaline phosphatase, and osteocalcin, together with collagen type III, TP-1, and TP-3, two epitopes of an osteoblastic differentiation marker. The data suggest that the effects of EMFs on osteoblastic differentiation are comparable to those of TGF-β and IGF-I. We conclude that EMF effects in the treatment of skeletal disorders and in orthopedic adjuvant therapy are mediated via enhancement of collagen type I mRNA expression, which may lead to extensive extracellular matrix synthesis. Bioelectromagnetics 19:222-231, 1998. © 1998 Wiley-Liss, Inc.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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