ISSN:
1420-9071
Keywords:
Molting hormone
;
ecdysteroids
;
3-dehydroecdysone
;
3α-hydroxyecdysteroids
;
Y-organ
;
crayfish
;
Procambarus clarkii
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract The injection of 3-dehydroecdysone (3dhE, 5 μ/g), the major ecdysteroid secreted by the Y-organ of crayfishProcambarus clarkii, resulted in apolysis within about 5 days. The hormonal response at the molecular level was investigated by injection of the radio-labeled compound; within 3 h of injection of [3H]3dhE, most radio-isotope was found in the extracted epidermal tissues and identified as ecdysone, 20-hydroxyecdysone (20E), and their 3α-hydroxy epimers. The biotransformation was undoubtedly performed in the peripheral area of the Y-organ. Cleavage of the polar conjugates, using an enzyme fromHelix pomatia, gave all of the above ecdysteroids including 3dhE. It was also found that the biosynthetic site of 3dhE was different from that of ecdysone at the subcellular level of the Y-organ.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01929922
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