ISSN:
1573-5001
Keywords:
3D NMR spectroscopy
;
Refocused HNCA quadruple resonance
;
Modified INEPT subsequence
;
HNCα correlations
;
Synchronous1H broadband decoupling
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Summary A 3D optimized, refocused HNCA experiment is described. It is demonstrated to yield a dramatic increase in sensitivity when applied to [13C,15N]-labeled human carbonic anhydrase II, a 29-kDa protein. The reasons for the gain in sensitivity are discussed, and 3 distinct areas for further development are indicated.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01875530
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