ISSN:
1432-2242
Keywords:
Keywords Rice
;
Stripe disease resistance gene
;
Stvb-i
;
BAC
;
Physical map
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The Stvb-i gene confers stripe disease resistance to rice. For positional cloning, we constructed a physical map spanning 1.8-cM distance between flanking markers, consisting of 18 bacterial artificial chromosome (BAC) clones, around the Stvb-i locus on rice chromosome 11. The 18 clones were isolated by screening a BAC library derived from a japonica cultivar, Shimokita, with three Stvb-i-linked RFLP markers and DraI-digested DNAs of a yeast artificial chromosome (YAC) clone. The results of Southern hybridization and restriction enzyme analyses indicated that these BAC clones are contiguous and cover about a 700-kb region containing the Stvb-i allele. Utilizing end and internal fragments of the BAC insert DNAs, 33 molecular markers were generated within a small chromosomal region including the Stvb-i locus. Genotyping analysis with these markers for a resistant cultivar and four nearby recombinants selected from 120 F2 individuals indicated that Stvb-i is contained within an approximately 286-kb region covered with two overlapping BAC clones.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s001220051449
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