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  • 1985-1989  (5)
  • Chemistry  (4)
  • 5-enolpyruvylshikimic acid  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Expression of glyphosate resistance in carrot somatic hybrid cells through the transfer of an amplified 5-enolpyruvylshikimic acid-3-phosphate synthase gene (1988)
    Springer
    Molecular genetics and genomics 211 (1988), S. 357-363 
    Details
    ISSN: 1617-4623
    Keywords: Gen amplification ; Glyphosate resistance ; 5-enolpyruvylshikimic acid ; 3-phosphate synthase (EPSPS) ; Protoplast fusion ; Amino acid analogs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A Daucus carota cell line selected as resistant to N-(phosphonomethyl)-glycine (glyphosate) was found to have increased levels of 5-enolpyruvylshikimic acid-3-phosphate synthase (EPSPS) activity of 5.5 times over wild-type carrot and an EPSPS protein level increase of 8.7 times as confirmed by Western hybridization analysis. Southern blot hybridization using a petunia EPSPS probe showed increases in the number of copies of EPSPS genes in the glyphosate-resistant line which correlated with the higher levels of the EPSPS enzyme. The mechanism of resistance to glyphosate is therefore due to amplification of the EPSPS gene. To examine the stability of the amplified genes, cloned lines selected as doubly resistant to Dl-5-methyltryptophan (5MT) and azetidine-2-carboxylate (A2C) were fused with the amplified EPSPS glyphosate-resistant cell line. Somatic hybrids expressed resistances to 5MT in a semidominant fashion while A2C and glyphosate resistance was expressed as dominant, or semi-dominant traits, in a line-specific manner. The hybrid lines possessed additive chromosome numbers of the parental lines used and no double minute chromosomes were observed. The glyphosate-resistant parental line and most somatic hybrids retained the amplified levels of EPSPS in the absence of selection pressure over a 3-year period.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00330616
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  • 2
    Electronic Resource
    Electronic Resource
    Stable isotope ratio analysis of leucine and ketoisocaproic acid in blood plasma by gas chromatography/mass spectrometry. Use of tertiary butyldimethylsilyl derivatives (1988)
    New York, NY : Wiley-Blackwell
    Rapid Communications in Mass Spectrometry 2 (1988), S. 14-16 
    Details
    ISSN: 0951-4198
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/rcm.1290020105
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Pressure losses in the packing stage of injection molding (1986)
    Stamford, Conn. [u.a.] : Wiley-Blackwell
    Polymer Engineering and Science 26 (1986), S. 1282-1289 
    Details
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The pressure loss between the mold and the nozzle in the injection molding of bar and box moldings has been monitored. The pressure drop observed during filling of the mold is reduced during the packing stage but remains finite. This has been attributed in the literature to solidification of polymer across the cavity transducer and to melt relaxation phenomena. Experiments have been carried out with hot molds to prolong the packing stage at the expense of the ‘cooling’ stage. Under these circumstances the pressure drop is reduced but not eliminated. The observed pressure drop may be related to the viscosity of the melt and its dependence on pressure and temperature although strain-induced crystallization and the pressure dependence of the melting point can confer effects similar to the cooling stage.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pen.760261808
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  • 4
    Electronic Resource
    Electronic Resource
    Pumping characteristics of self-wiping twin-screw extruders - A theoretical and experimental study on biopolymer extrusion (1989)
    Stamford, Conn. [u.a.] : Wiley-Blackwell
    Polymer Engineering and Science 29 (1989), S. 433-440 
    Details
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The flow of Newtonian and non-Newtonian fluids which obeys a power law relationship between shear stress and shear rate has been modeled in the melt conveying section of a self-wiping co-rotating twin-screw extruder using a finite element analysis of an unwound channel section. Predictions of throughput against pressure gradient are compared with experimentally obtained results for maize grits which is represented as a power law material. Rheological data applicable to extrusion simulation were obtained from capillary rheometry. Comparisons are reasonable with predicted characteristic showing similar behavior.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pen.760290702
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  • 5
    Electronic Resource
    Electronic Resource
    Some features of the injection molding of short fiber reinforced thermoplastics in center sprue-gated cavities (1987)
    Brookfield, Conn. : Wiley-Blackwell
    Polymer Composites 8 (1987), S. 16-21 
    Details
    ISSN: 0272-8397
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: This paper describes an investigation into the fiber orientation in a number of center sprue fed cavities in short glass fiber filled polypropylene and nylon. The data have been interpreted in terms of a generalized five-layer structure resulting from the frozen skin formation and the high and low shear levels in the flowing melt. The implications for scaling up the mold size are discussed from the results obtained with different shot volumes. The fiber structure was observed to depend on location in the molding, local injection time, and injection rate. In addition the occurrence of fiber-free layers within the moldings using the filled polypropylene increases with an increase in shot volume, which produces an inherent ‘scale-up’ problem. Notwithstanding the mold geometry subtleties, the fiber orientation in all the moldings follows similar patterns and trends.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pc.750080104
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    Paper (German National Licenses)
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