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  • 1
    ISSN: 1432-0630
    Keywords: 89.60 ; 35.80 ; 06.70
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract Laser heterodyne spectroscopy (LHS) techniques with semiconductor laser local oscillators (LO) in the 3–30 μm range have the potential to measure radical gas species in the stratosphere. The goal of this experiment is to measure radical gases from Spacelab, including ClO, ClONO2, HO2, H2O2, N2O5, and HOCl in solar occulation with vertical resolution ≦2km and vertical range from 10 to 70 km. Sensitivity analyses have been performed on ClO and O3 to determine design criteria for the LHS instrument. Results show that O3 and ClO vertical profiles can be measured with an accuracy ≧95% and ≧80%, respectively, over the total profile. These accuracies require the LO to maintain the following characteristics: frequency stability (Δf w≦20 MHz), single-mode power (P LO≧500 μW), and minimum frequency drift (≦5 MHz). Laboratory heterodyne measurements performed with semiconductor lasers generated the same shot-noise photocurrent as CO2 lasers, for comparable single-mode power. “Excess-noise” regions were identified, but could be wavelength controlled by fine control of operating temperature and injection current. Doppler-shift effects and limited solar occultation measurement times due to Spacelab orbits should pose minimum mission constraints on the experiment.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1435-1803
    Keywords: Na,K-ATPase ; 3H-ouabain binding ; heart failure ; tachycardia ; potassium ; calcium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The present study evaluates3H-ouabain binding site (Na,K-ATPase) concentration in left ventricular myocardium of dogs with heart failure induced by tachycardia as a result of ventricular pacing. Samples of left ventricle were obtained from 10 dogs exposed to pacing of 240 beats/min for 3 to 4 weeks and eight sham-operated controls. Na,K-ATPase was quantified using vanadate facilitated3H-ouabain binding to intact samples. At time of sacrifice paced dogs showed clinical signs of heart failure, a significant 257% increase in left ventricular end diastolic pressure and a significant 46% decrease in left ventricular dP/dt compared with control. There was no significant change in left ventricular mass.3H-ouabain binding concentration was significantly reduced by 16%. Evaluation of3H-ouabain binding kinetics revealed no significant difference between myocardium from paced and control dogs: Equilibrium binding conditions were at the various concentrations used obtained after similar incubation time; nonspecific uptake and retention of3H-ouabain was 0.9–0.8% of total uptake and retention obtained in the standard assay; apparent dissociation constant (KD) was 6.5×10−8–6.6×10−8mol/l; loss of specifically bound3H-ouabain during washout at 0°C occurred with a half-life time (T3/2) of 120 and 121h. Hence, total3H-ouabain binding site concentration in left ventricular myocardium was (mean±SEM) 1110±56 and 1317±68 pmol/g wet weight, 8.54±0.43 and 10.05±0.52 pmol/mg protein, and the total amount of3H-ouabain binding sites in the entire left ventricle 121±6 and 162±8 nmol in paced (n=10) and control (n=8) dogs (p〈0.05), respectively. In conclusion, the present study reports a significant reduction in left ventricular myocardium3H-ouabain binding site concentration in tachycardia induced heart failure. This observation supports the concept of a relationship between Na,K-ATPase concentration and contractile capacity and may be of pathophysiological importance in tachycardia and heart failure.
    Type of Medium: Electronic Resource
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