ISSN:
1432-1424
Keywords:
Calcium-activated nonselective channel
;
Rat insulinoma cell line
;
CRI-G1
;
Pyridine nucleotides β-NAD+-NS+ channel
;
Nucleotide regulation
;
AMP
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Abstract The activity of a calcium-activated nonselective (Ca-NS+) channel in a rat insulinoma cell line (CRI-G1) is inhibited by pyridine nucleotides in excised patches. The effects of all four pyridine nucleotides tested, β-NAD+, β-NADH, β-NADP+ and β-NADPH were very similar when tested at 0.1 mm, and at 1 mm the phosphorylated forms, β-NADP+ and β-NADPH, appeared to be slightly more potent than β-NAD+ and β-NADH. All the pyridine nucleotides tested reduced both the open state probability of the channel and the number of functional channels observed in a single patch. The application of β-NAD+, but not of the other nucleotides tested, to the cytoplasmic surface of isolated inside-out patches from CRI-G1 cells opened a novel nonselective cation channel (the β-NAD+-NS+ channel). The activity of this new channel is calcium sensitive and may also be inhibited by AMP.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00233437
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