ISSN:
1432-203X
Keywords:
Abbreviations: BMS cells: Black Mexican Sweet cells, GUS: β‐glucuronidase, CAT: chloramphenicol acetyltransferase, PAT: Phoshinotricin acetyltransferase, MS: Murashige and Skoog, PVC: packed cell volume, 4‐MU and 4‐MUG: 4‐methylumbelliferone and 4‐methylumbelliferyl‐glucuronide, BSA: bovine serum albumin, TTC: triphenyl tetrazolium chloride
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary. When interested in plant cell transformation, the cell wall is often considered as a barrier to DNA transfer, which is only overcome by wounding or wall degrading enzymes. In this work, we demonstrate that cell plasmolysis before electropulsation is an efficient approach to DNA delivery into intact plant cells. Using such a method, transient expression (β‐glucuronidase and chloramphenicol acetyltransferase) and stable expresion (phosphinotricin acetyltransferase) of exogenous genes are obtained in intact black Mexican sweet maize cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990050150
Permalink
