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  • 1
    Electronic Resource
    Electronic Resource
    The effect of cytochalasin B on the rate of growth and ultrastructure of wheat coleoptiles and maize roots (1979)
    Springer
    Planta 144 (1979), S. 373-383 
    Details
    ISSN: 1432-2048
    Keywords: Auxin ; Cell elongation ; Cytochalasin B ; Dictyosomes ; Secretion ; Triticum ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cytochalasin B (CB) inhibits the elongation growth of maize roots, and that of wheat coleoptile segments incubated in indolyl-3-acetic acid, by over 30% after a lag period of about 60 min. This long lag is not due to poor tissue penetration by the inhibitor, but seems to reflect a property of the process inhibited by CB. The only visible ultrastructural change accompanying growth inhibition is the accumulation of secretory vesicles in the vicinity of dictyosomes, which occurs between 90 and 300 min. However, a massive accumulation of vesicles is seen after 120 min in root cap cells which possess very active dictyosomes. The results indicate that CB does not inhibit elongation growth by interfering with cytoplasmic streaming. Instead, they indicate that the drug acts to inhibit the secretion of cell wall components at some stage after vesicle production, but prior to their transport.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00391581
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Characterization of solute transport in plasma membrane vesicles isolated from cotyledons ofRicinus communis L. (1990)
    Springer
    Planta 182 (1990), S. 532-539 
    Details
    ISSN: 1432-2048
    Keywords: ATPase ; Plasma membrane ; Pyrophosphatase ; Ricinus (solute transport) ; Sucrose transport
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A highly enriched plasma membrane fraction has been isolated from dark-grown cotyledons ofRicinus communis by phase partitioning. This is demonstrated by the properties of the associated ATPase: high vanadate sensitivity, azide and nitrate insensitivity, sharp pH optimum around 6.5, and high specificity for ATP as substrate. The upper plasma membrane fraction also contained a pyrophosphatase activity, normally considered to be located on the tonoplast or Golgi membranes, which showed a specific activity higher than that in the lower phase. Sucrose gradient centrifugation of both microsomal and upper phase fractions showed a comigration of some pyrophosphatase activity with the plasma membrane fraction. Sucrose uptake changes with development inRicinus cotyledons. The ATPase activity in the upper (plasma membrane) phase also varied in a similar way with development, whereas activity in the lower phase showed little change. Pyrophosphatase activity in the upper phase also increased with development but did not show a peak and fall as seen for sucrose uptake and ATPase. The possibility that changes in plasma membrane ATPase may contribute to changes in sucrose uptake capacity and the possible cellular origin and physiological significance of the pyrophosphatase activity are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02341028
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    The role of the epidermis in auxin-induced and fusicoccin-induced elongation of Pisum sativum stem segments (1980)
    Springer
    Planta 150 (1980), S. 371-379 
    Details
    ISSN: 1432-2048
    Keywords: Auxin ; Cell elongation ; Epidermis peeling ; Fusicoccin ; Pisum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effects of peeling and wounding on the indole-3-acetic acid (IAA) and fusicoccin (FC) growth response of etiolated Pisum sativum L. cv. Alaska stem tissue were examined. Over a 5 h growth period, peeling was found to virtually eliminate the IAA response, but about 30% of the FC response remained. In contrast, unpeeled segments wounded with six vertical slits exhibited significant responses to both IAA and FC, indicating that peeling does not act by damaging the tissue. Microscopy showed that the epidermis was removed intact and that the underlying tissue was essentially undamaged. Neither the addition of 2% sucrose to the incubation medium nor the use of a range of IAA concentrations down to 10-8 M restored IAA-induced growth in peeled segments, suggesting that lack of osmotic solutes and supra-optimal uptake of IAA were not important factors over this time period. It is concluded that, although the possibility remains that peeling merely allows leakage of hydrogen ions into the medium, it seems more likely that peeling off the epidermis removes the auxin responsive tissue.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00390172
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  • 4
    Electronic Resource
    Electronic Resource
    Cytochemical localization of plasma membrane ATPase activity in plant cells (1991)
    Springer
    Protoplasma 165 (1991), S. 27-36 
    Details
    ISSN: 1615-6102
    Keywords: ATPase ; Cerium ; Cytochemistry ; Fixation ; Zea mays roots ; Plasma membrane
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The cytochemical localization of ATPase activity has been investigated in maize root cells using both lead and cerium-based capture methods. With both methods, staining at the plasma membrane was observed in all cells of the root, although the precipitate obtained with cerium was more uniform and granular than that with lead. Controls using no substrate or no magnesium, β-glycerophosphate to replace ATP, vanadate or boiled tissue generally showed little or no staining. However, biochemical studies on purified plasma membrane fractions showed that ATPase activity was markedly inhibited by fixation, particularly by glutaraldehyde, and also by lead and cerium ions. Non-enzymic hydrolysis of ATP by cerium was greater than that by lead. The value and limitations of these procedures for the localization of plasma membrane H+-ATPase activity are summarized in relation to previous criticisms of these methods.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01322274
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    Paper (German National Licenses)
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