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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Journal of chemical ecology 16 (1990), S. 2713-2722 
    ISSN: 1573-1561
    Keywords: Monoterpenes ; Abies grandis ; Picea pungens ; steam distillation ; extraction ; secondary chemicals ; grand fir ; blue spruce ; coefficient of variation ; MANOVA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Five extraction techniques were used to obtain quantitative measurements of monoterpenes from blue spruce and grand fir needles. Multivariate analysis of variance indicated that there was a significant difference in the amount of each monoterpene extracted by using different techniques. For both species, an 8-hr steam distillation of 10 g of material yielded the greatest amount of each monoterpene. This technique was also the most reproducible in that the coefficients of variation were lowest. A rapid steam distillation was relatively effective for grand fir, but not for blue spruce. A CO2 extraction was unreliable for both species.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-1561
    Keywords: Damson-hop aphid ; Phorodon humuli ; Homoptera ; Aphididae ; methyl salicylate ; (E)-2-hexenal ; β-caryophyllene ; semiochemicals ; aggregation ; attractant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Behavioral studies using an olfactometer demonstrated that spring migrants of the damson-hop aphid,Phorodon humuli, respond to semiochemicals released by spring migrants feeding on hop leaves. Samples of the total volatiles released on feeding were analyzed by coupled gas chromatographysingle cell recording techniques and showed the presence of several active components. Three compounds were identified, using coupled gas chromatography-mass spectrometry, as methyl salicylate, (E)-2-hexenal andβ-caryophyllene. These three compounds elicited responses from separate olfactory receptors on the antenna. In the olfactometer, both (E)-2-hexenal andβ-caryophyllene gave positive responses from spring migrants, and a mixture of the two compounds in the natural ratio was more attractive than (E)-2-hexenal alone. Addition of methyl salicylate eliminated the response to the active binary mixture.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-1561
    Keywords: Pheromone ; sex ; attractant ; aphid ; Phorodon humuli ; Homoptera ; Aphididae ; nepetalactol ; monoterpenoid ; electrophysiology ; single cell recording ; mass spectrometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Behavioral studies using an olfactometer demonstrated that sexual females (oviparae) of the damson-hop aphid,Phorodon humuli, release a pheromone to which males respond. Volatiles produced by the oviparae were analyzed by coupled gas chromatography-single cell recording from the secondary rhinaria on the male antenna and showed the presence of one peak with major activity. Coupled gas chrornatography-mass spectrometry suggested a nepetalactol, which was shown to have the 4aR, 7S, 7aS stereochemistry by synthesis from the corresponding nepetalactone isolated from the labiate plantNepeta mussinii. Although the stereochemistry at carbon-1 is not yet established, a synthetic sample comprising ca. 70% 1S and 30% 1R attracted highly significant numbers of males to water traps placed within and adjacent to a hop garden. Initial studies also indicated attraction of males in both the olfactometer and in the field by volatiles from the primary host.
    Type of Medium: Electronic Resource
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