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  • 1
    Digitale Medien
    Digitale Medien
    Four polymorphic markers on rat chromosome 12 form a single linkage group (1993)
    Springer
    Biochemical genetics 31 (1993), S. 441-448 
    Details
    ISSN: 1573-4927
    Schlagwort(e): linkage analysis ; serine dehydratase ; plasminogen activator inhibitor ; simple sequence repeats ; microsatellites ; rat chromosome 12
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Four PCR-typable polymorphic markers were mapped to rat chromosome 12 by linkage analysis of F2 intercross progeny of Fischer (F344/N) and Lewis (LEW/N) rat strains. The markers formed a single linkage group, covering 27.7 cM, with the following order and distance between markers: plasminogen activator inhibitor (Planh)—0.0 cM—phosphoenolpyruvate carboxykinase-related sequence 2 (Pepckr2)—15.4 cM—anonymous marker (D12N155)—12.3 cM—serine dehydratase (Sdh). All markers were identified and genotyped by PCR analysis of simple sequence repeats. The gene encoding Planh was previously assigned to rat chromosome 12, which allowed us to assign the entire linkage group to this chromosome. These markers were highly polymorphic in 13 additional inbred rat strains (BUF/N, BN/SsN, WKY/N, MNR/N, LER/N, WBB1/N, WBB2/N, MR/N, LOU/MN, SHR/N, ACI/N, SR/Jr, and SS/Jr). These markers should be useful tools for further genetic studies in rats.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00553460
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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    Artikel (Nationallizenzen)
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  • 2
    Digitale Medien
    Digitale Medien
    Four polymorphic markers on rat chromosome 12 form a single linkage group (1993)
    Springer
    Biochemical genetics 31 (1993), S. 441-448 
    Details
    ISSN: 1573-4927
    Schlagwort(e): linkage analysis ; serine dehydratase ; plasminogen activator inhibitor ; simple sequence repeats ; microsatellites ; rat chromosome 12
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Four PCR-typable polymorphic markers were mapped to rat chromosome 12 by linkage analysis of F2 intercross progeny of Fischer (F344/N) and Lewis (LEW/N) rat strains. The markers formed a single linkage group, covering 27.7 cM, with the following order and distance between markers: plasminogen activator inhibitor (Planh)—0.0 cM—phosphoenolpyruvate carboxykinase-related sequence 2 (Pepckr2)—15.4 cM—anonymous marker (D12N155)—12.3 cM—serine dehydratase (Sdh). All markers were identified and genotyped by PCR analysis of simple sequence repeats. The gene encoding Planh was previously assigned to rat chromosome 12, which allowed us to assign the entire linkage group to this chromosome. These markers were highly polymorphic in 13 additional inbred rat strains (BUF/N, BN/SsN, WKY/N, MNR/N, LER/N, WBB1/N, WBB2/N, MR/N, LOU/MN, SHR/N, ACI/N, SR/Jr, and SS/Jr). These markers should be useful tools for further genetic studies in rats.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02396228
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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    Artikel (Nationallizenzen)
    Volltext
  • 3
    Digitale Medien
    Digitale Medien
    Ultrastructural and immunochemical analyses of the distribution of microfilaments in seedlings and plants ofGlycine max (1984)
    Springer
    Protoplasma 120 (1984), S. 91-99 
    Details
    ISSN: 1615-6102
    Schlagwort(e): Actin ; Glycine max ; Microfilaments ; Soybean
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Filamentous structures were observed when cytoplasmic extracts of various tissues of soybean plants and seedlings were examined by electron microscopy. Three main lines of evidence indicate that these structures represented microfilaments derived from the soybean tissues: a) the diameter of the filaments was estimated to be 6–7 nm; b) the addition of rabbit heavy meromyosin resulted in the decoration of the filaments, yielding characteristic arrow-head patterns; and c) ATP reversed the decoration of the filaments by heavy meromyosin. When the various anatomical parts of soybean plants and seedlings were compared for the presence of microfilaments, the root tips and radicles showed the highest frequency while the petioles and cotyledons yielded no observable filaments. In order to substantiate these findings, a quantitative radioimmunoassay was developed using rabbit antibodies directed against calf thymus actin. These studies demonstrated that the concentration of actin in extracts of the root tip was 15-fold higher than in those of the petiole and leaf. Similar comparisons of various parts of soybean seedlings showed that the radicle was rich in actin. These results suggest that actin filaments are found predominantly in the subterranean parts of plants.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01287621
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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    Artikel (Nationallizenzen)
    Volltext
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