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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Journal of statistical physics 57 (1989), S. 1069-1098 
    ISSN: 1572-9613
    Keywords: Block copolymers ; generating function ; Polya's theorem ; gelation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract We determine the statistical properties of block copolymer complexes in solution. These complexes are assumed to have the topological structure of (i) a tree or of (ii) a line-dressed tree. In case the structure is that of a tree, the system is shown to undergo a gelation transition at sufficiently high polymer concentration. However, if the structure is that of a line-dressed tree, this transition is absent. Hence, we show the assumption about the topological structure to be relevant for the statistical properties of the system. We determine the average size of the complexes and calculate the viscosity of the system under the assumption that the complexes geometrically can be treated as porous spheres.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Journal of statistical physics 57 (1989), S. 1099-1122 
    ISSN: 1572-9613
    Keywords: Block copolymers ; generating function method ; Polya's theorem ; gelation ; nonnested structures
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract We determine the statistical properties of block copolymers in solution. These complexes are assumed to have the topological structure of connected graphs with “nonnested” loops and cycles. The generating function method is used to determine the number of topologically different complexes containing a given number of block copolymers. It is shown that at sufficiently high concentration the system undergoes a transition to a gel phase. Furthermore, the average number of polymers per complex is calculated. Finally, the relative increase in viscosity is found under the assumption that the complexes can be treated as porous spheres.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1617-4623
    Keywords: Tomato ; Agrobacterium T-DNA ; Inverse PCR ; Activator ; Transposon tagging
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We are developing a system for isolating tomato genes by transposon mutagenesis. In maize and tobacco, the transposon Activator (Ac) transposes preferentially to genetically linked sites. To identify transposons linked to various target genes, we have determined the RFLP map locations of Ac- and Dissociation (Ds)-carrying T-DNAs in a number of transformants. T-DNA flanking sequences were isolated using the inverse polymerase chain reaction (IPCR) and located on the RFLP map of tomato. The authenticity of IPCR reaction products was tested by several criteria including nested primer amplification, DNA sequence analysis and PCR amplification of the corresponding insertion target sequences. We report the RFLP map locations of 37 transposon-carrying T-DNAs. We also report the map locations of nine transposed Ds elements. T-DNAs were identified on all chromosomes except chromosome 6. Our data revealed no apparent chromosomal preference for T-DNA integration events. Lines carrying transposons at known map locations have been established which should prove a useful resource for isolating tomato genes by transposon mutagenesis.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1573-9368
    Keywords: transposon ; Activator ; Dissociation ; cell-autonomous ; spectinomycin ; tomato ; phosphinothricin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cell-autonomous genes have been used to monitor the excision of both endogenous transposons in maize andAntirrhinum, and transposons introduced into transgenic plants. In tobacco andArabidopsis, the streptomycin phosphotransferase (SPT) gene reveals somatic excision of the maize transposonActivator (Ac) as green sectors on a white background in cotyledons of seedlings germinated in the presence of streptomycin. Cotyledons of tomato seedlings germinated on streptomycin-containing medium do not bleach, suggesting that a different assay for transposon excision in tomato is desirable. We have tested the use of the spectinomycin resistance (SPEC) gene (aadA) and a Basta resistance (BAR) gene (phosphinothricin acetyltransferase, or PAT) for monitoring somatic excision ofAc in tobacco and tomato. Both genetic and molecular studies demonstrate that genotypically variegated individuals that carry clones of cells from whichAc orDs have excised from either SPEC or BAR genes, can be phenotypically completely resistant to the corresponding antibiotic. This demonstrates that these genes act non-cell-autonomously, in contrast to the SPT gene in tobacco. Possible reasons for this difference are discussed.
    Type of Medium: Electronic Resource
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