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  • 1
    Electronic Resource
    Electronic Resource
    Adenine requirement for nodulation of pea by an auxotrophic mutant of Rhizobium leguminosarum (1974)
    Springer
    Archives of microbiology 100 (1974), S. 219-238 
    Details
    ISSN: 1432-072X
    Keywords: Rhizobium ; Nodulation ; Symbiosis ; Adenine ; Thiamine ; Auxotroph ; Prototroph
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A non-nodulating auxotroph (L4-73) derived from an effective strain (L4) of Rhizobium leguminosarum has a growth requirement for adenine and thiamine. The auxotroph was able to infect the roots of the host plant Pisum sativum L. but formed root nodules (ineffective in nitrogen fixation) only when adenine and, to a lesser extent, thiamine were added to the plant substrate. Nodules formed in the presence of adenine were structurally abnormal, containing small cells in which infection threads appeared to have aborted. In the presence of thiamine the auxotroph produced a smaller number of nodules which were slightly more developed and were able to reduce trace amounts of acetylene to ethylene. The adenine effect predominated when both growth factors were added together or separately in different sequences. Adenine amendment was required during the first 6 days following inoculation to achieve the maximum number of nodules. The block in symbiosis could not be fully overcome by sequential addition or removal from the root medium of either compound or by addition of some other adeninecontaining compounds. Partial prototroph revertants requiring adenine but not thiamine induced a nodulation response similar to that of the original auxotroph in the presence of thiamine; partial prototrophs requiring thiamine only were almost fully effective. Bioassay of pea root tissue indicated the presence of significant amounts of both adenine and thiamine or related substances in the roots. The auxotroph was able to compete with the parent strain L4 in nodulation on roots of pea only in the presence of exogenous adenine.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00446319
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Carnitine: a novel compatible solute in Lactobacillus plantarum (1994)
    Springer
    Archives of microbiology 162 (1994), S. 243-248 
    Details
    ISSN: 1432-072X
    Keywords: Key words     Lactobacillus plantarum ; Compatible ; solute ; Osmotic stress ; Carnitine ; Betaine ; Acetylcholine ; Succinylcholine ; γ-Butyrobetaine ; Quaternary ammonium compound
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract      The aim of this study was to unravel the identity of compatible solutes accumulated by Lactobacillus plantarum subjected to osmotic stress. Betaine was accumulated simultaneously with a novel compatible solute identified as carnitine, both present in the complex medium applied in this study. Beef extract provided the main source of carnitine in the medium. Both carnitine and betaine were accumulated to maximum concentrations of 248 and 231 μmol.g dry weight–1, respectively. A defined medium was devised devoid of carnitine. Addition of 0.5 mM carnitine to this medium increased the growth rate from 0.1 h–1 to 0.2 h–1 in media with 0.4 M sodium chloride. Also, carnitine made the organism more tolerant to sodium chloride. Growth occurred even when the sodium chloride concentration was raised from 0.5 M to 1.0 M. Quaternary compo unds resembling the structure of carnitine and betaine enhanced the growth yield as well. γ-Butyrobetaine and succinylcholine restored the growth yield up to respectively 91 and 96% compared to non-stressed cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00301845
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Carnitine: a novel compatible solute in Lactobacillus plantarum (1994)
    Springer
    Archives of microbiology 162 (1994), S. 243-248 
    Details
    ISSN: 1432-072X
    Keywords: Lactobacillus plantarum ; Compatible solute ; Osmotic stress ; Carnitine ; Betaine ; Acetylcholine ; Succinylcholine ; γ-Butyrobetaine ; Quaternary ammonium compound
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The aim of this study was to unravel the identity of compatible solutes accumulated by Lactobacillus plantarum subjected to osmotic stress. Betaine was accumulated simulataneously with a novel compatible solute identified as carnitine, both present in the complex medium applied in this study. Beef extract provided the main source of carnitine in the medium. Both carnitine and betaine were accumulated to maximum concentrations of 248 and 231 μmol.g dry weight-1, respectively. A defined medium was devised devoid of carnitine. Addition of 0.5 mM carnitine to this medium increased the growth rate from 0.1 h-1 to 0.2 h-1 in media with 0.4 M sodium chloride. Also, carnitine made the organism more tolerant to sodium chloride. Growth occurred even when the sodium chloride concentration was raised from 0.5 M to 1.0 M. Quaternary compounds resembling the structure of carnitine and betaine enhanced the growth yield as well. γ-Butyrobetaine and succinylcholine restored the growth yield up to respectively 91 and 96% compared to non-stressed cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00301845
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
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