ISSN:
1432-1912
Keywords:
Patch clamp
;
Adenosine triphosphate-sensitive potassium channel
;
Cardiotonic agent
;
Skeletal muscle
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary The inhibition of ATP-sensitive potassium channels in mouse skeletal muscle by the cardiotonic bipyridine AWD 122-60 was investigated with the patch-clamp technique. In excised patches of the inside-out configuration, internally applied AWD 122-60 (10−6−10−3 mol/1) reversibly reduced the open-probability of single ATP-sensitive potassium channels. The agent shortened the periods of channel activity but did not affect the channel conductance. At positive membrane potentials channel inhibition by AWD 122-60 was more pronounced than at negative potentials, the drug concentrations producing 50% channel inhibition were 11 μmol/l at + 40 mV and 29 μmol/l at −40 mV. The Hill coefficients of the concentration-response curves were in the range between 0.5 and 0.6 for both potentials. milrinone (10−4 mol/I), had no effects on ATP-sensitive potassium channels in skeletal muscle. potassium channels in heart muscle by AWD 122-60 are discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00168951
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