ISSN:
1617-4623
Keywords:
Lycopersicon esculentum
;
Ethyl methanesulphonate (EMS) treatment
;
Allyl alcohol selection
;
Alcohol dehydrogenase (ADH)
;
Adh-1 null mutant
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Treatment of tomato seeds with ethyl methanesulphonate (EMS) followed by allyl alcohol selection of M2 seeds has led to the identification of one plant (B15-1) heterozygous for an alcohol dehydrogenase (Adh) null mutation. Genetic analysis and expression studies indicated that the mutation corresponded to the structural gene of the Adh-1 locus on chromosome 4. Homozygous Adh-1 null mutants lacked ADH-1 activity in both pollen and seeds. Using an antiserum directed against ADH from Arabidopsis thaliana, which crossreacts with ADH-1 and ADH-2 proteins from tomato, no ADH-1 protein was detected in seeds of the null mutant. Northern blot analysis showed that Adh-1 mRNA was synthesized at wild-type levels in immature seeds of the null mutant, but dropped to 25% in mature seeds. Expression of the Adh-2 gene on chromosome 6 was unaffected. The potential use of the Adh-1 null mutant in selecting rare transposon insertion mutations in a cross with “mutable” Adh-1 + tomato lines is discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00273595
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