ISSN:
1432-072X
Keywords:
Keywords Denitrification
;
Pseudomonas putida
;
Nitrate reductase
;
Periplasm
;
Aerobic nitrate respiration
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A strain of Pseudomonas putida that can express a nitrate reductase that is located in the periplasmic compartment was isolated from freshwater. The enzyme was active in vivo during arginine fermentation and at the onset of oxygen limitation in batch cultures. The activity of the enzyme increased the yield of bacteria following fermentative growth under anoxic conditions with arginine, but nitrate reduction did not support growth on non-fermentable carbon substrates under anoxic conditions. Cells expressing the periplasmic nitrate reductase were capable of reducing nitrate in the presence of oxygen. Nitrate reduction under oxic conditions was clearly coupled to a respiratory electron transport chain because: (1) the process was sensitive to the respiratory inhibitors rotenone and 2-n-heptyl-4-hydroxyquinoline N-oxide, and (2) membrane-bound and periplasmic cytochromes were involved. This is the first report of the presence of a periplasmic nitrate reductase in a member of the γ proteobacteria.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00305348
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