ISSN:
1612-1112
Schlagwort(e):
Affinity chromatography
;
Bovine serum albumin
;
Competitive drug binding
;
SDS detergent
;
Hummel-Dreyer method
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Chemie und Pharmazie
Notizen:
Summary The competitive binding of five drugs and a detergent to bovine serum albumin at pH 7.4 and room temperature was studied by Hummel-Dreyer method in high performance liquid chromatography. The five drugs are: warfarin, sulfinpyrazone, aspirin, quinidine gluconate and lidocaine and the detergent is the sodium dodecyl sulfate (SDS). While the quantitative techniques of Hummel-Dreyer method have been well developed during last twenty years, this paper reports, perhaps for the first time, the qualitative techniques of Hummel-Dreyer method as an analytic tool to ascertain whether a drug would bind to protein and how one drug would affect another drug in binding, if the binding does occur. The results on the basis of qualitative observation indicate the strength of binding in the following order: warfarin 〉 aspriin 〉 lidocaine 〉 sulfinpyrazone 〉 quinidine gluconate. The SDS has capacity to disturb the binding site on the surface of protein.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF02278643
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