ISSN:
1612-1112
Schlagwort(e):
Column liquid chromatography
;
Affinity chromatography
;
Anhydrotrypsin
;
C-terminal Arg- or Lys-containing peptides
;
Diol silica
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Chemie und Pharmazie
Notizen:
Summary Anhydrotrypsin (AHT), a catalytically inert derivative of trypsin in which the active site serine residue was converted to dehydroalanine residue by chemical modification, was immobilized onto diol silica through the activation with trifluoroethanesulfonyl chloride, and an AHT-diol-silica column was used for high-performance affinity chromatography separation of peptides containing arginine or lysine at their C-termini from the others. Improved separation in terms of speed was accomplished.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF02328507
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