ISSN:
1573-5028
Keywords:
Agrobacterium tumefaciens
;
adventitious shoot regeneration
;
transformation
;
homozygous potato
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Transformed potato (Solanum tuberosum) plants were obtained from homozygous diploid potato by using a transformation procedure in combination with an adventitious shoot regeneration method. Leaf and stem explants were inoculated with an Agrobacterium tumefaciens strain which contained a binary vector (pVU 1011) carrying the neomycin phosphotransferase gene. Shoot regeneration most effectively on stem explants, occurred within six weeks directly from the explants without introducing a callus phase. A strong seasonal influence on transformation efficiencies was observed. Analysis of a number of randomly selected regenerated shoots for their ability to root and form shoots on kanamycin-containing medium shows that over 90% of the regenerated shoots obtained are transformed. In a number of shoots transformation was confirmed by a test for the presence and expression of the NPT-II gene.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00043210
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