ISSN:
1476-5535
Keywords:
Metabolism
;
Acetate
;
Alginate
;
Carbon balance
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Summary Some metabolic properties of both suspended and immobilized aerobically and anaerobically growingEscherichia coli cells were investigated. Metabolic activity was found to be substantially different whenE. coli cells were immobilized in alginate. Cells grown immobilized in alginate, and then released from the gel, synthesized 1.6 (aerobic growth) and 4.9 (anaerobic growth) times as much β-galactosidase per cell in response to induction as did suspended cells. Under both aerobic and anaerobic conditions, the cell yield from glycerol for immobilized cells was half that for suspended cells. At specific growth rates that were not significantly different from those of suspended cells, immobilized cells consumed glycerol at twice the rate of suspended cells. Immobilized cells produced elevated quantities of acetate, pyruvate, and lactate. Interpretation of these findings is discussed in terms of the kinetics of energy metabolism and the regulation of inducible protein synthesis inE. coli.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01574081
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