ISSN:
1573-5044
Keywords:
abscisic acid
;
Allium cepa
;
Allium fistulosum
;
Allium porrum
;
MS medium
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The plant regeneration ability of zygotic embryo-derived callus cultures was studied for 12 A. cepa varieties and accessions, two A. fistulosum varieties, one A. fistulosum x A. cepa interspecific hybrid and two A. porrum varieties. Compact embryogenic callus was induced on Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxyacetic acid. The embryogenic calluses of all three Allium species were similar in appearance. For all accessions tested plants could be regenerated at a high frequency from this compact callus through somatic embryogenesis, when using kinetin supplemented MS medium (regeneration medium). Addition of abscisic acid to the regeneration medium stimulated the formation of both somatic embryos and shoots for a number of varieties. Concerning shoot regeneration from callus cultures, significant differences existed between genotypes of all accessions except one.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00040020
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