ISSN:
1052-9306
Keywords:
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
The positive ion fast atom bombardment (FAB) mass spectra of oligosaccharides related to D-xylan and D-glucurono-D-xylans were investigated. Although the mass spectra of the underivatized oligosaccharides are relatively weak, they still offer reliable information on the molecular weight of these compounds from their [M + H]+ and [M + H + glycerol]+ ions. Esterification, glycosidation and/or partial methylation improve the relative abundances of the molecular and fragment ions with respect to those of the glycerol matrix. The mass spectra of these derivatized compounds also allow the determination of the mass of the monosaccharide units by the occurrence of more abundant and characteristic fragment ions. The differently linked D-xylobioses of the (1 → 2), (1 → 3) and (1 → 4) type cannot be distinguished on the basis of their mass spectra alone. The collisional activation (CA) spectra of the [M + H + glycerol]+ adduct ions, however, revealed that an unequivocal determination of the type of linkage in these isomers is possible. The small values of the discrepancy factors in the FAB CA spectra prove that CA mass spectrometry is, also in combination with FAB ionization, the most precise of the current mass spectrometric techniques.
Additional Material:
3 Tab.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bms.1200170607
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