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  • Biochemistry and Biotechnology  (3)
  • Analytical Chemistry and Spectroscopy  (2)
  • steady flow  (2)
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Schlagwörter
  • 1
    Digitale Medien
    Digitale Medien
    Potentiometric method for subatrate analysis using immobilized NAD+-dependent oxidoreductase enzymes (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 21 (1979), S. 1905-1915 
    Details
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Two coenzyme-dependent oxidoreductases, glucose dehydrogenase and alcohol dehydrogenase, were immobilized in polyacrylamide gel over a platinum grid matrix and used as enzyme electrodes to measure their substrate concentrations in buffered aqueous solutions. The immobilized enzymes were used to oxidize their substrates in the presence of NAD+. Ferricyanide was used as the redox mediator and electroactive specific. The determinations of glucose and ethenol were utilized to demonstrate and evaluate the performance of the system. The described methodology should be readily applicable to the analysis of numerous other substrates of coenzyme-dependent oxidoreductases.
    Zusätzliches Material: 6 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/bit.260211103
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  • 2
    Digitale Medien
    Digitale Medien
    Potentiometric quantitation of glycerol using immobilized glycerol dehydrogenase (1982)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 24 (1982), S. 971-975 
    Details
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Glycerol dehydrogenase was immobilized in polyacrylamide gel layered over a small platinum screen and used to catalyze the oxidation of glycerol. In the presence of NAD+ and potassium ferricyanide, the coupling reaction generated a measurable electrical potential which was found to be Nernstian with respect to the glycerol concentration range of 10-4M to 10-1M. The reproducibility of the measurement and the optimal conditions for glycerol determination were described.
    Zusätzliches Material: 1 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/bit.260240416
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  • 3
    Digitale Medien
    Digitale Medien
    Electrochemical evaluation of lactate dehydrogenase immobilized in polyacrylamide gels (1977)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 19 (1977), S. 1785-1792 
    Details
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Lactate dehydrogenase (EC 1.1.1.27) has been immobilized in polyacrylamide gels over a platinum grid matrix. The immobilized enzyme is used to oxidize L-lactate in the presence of nicotinamide adenine dinucleotide (NAD+) and femcyanide. The NADH produced is then chemically oxidized back to NAD+by ferricyanide. The coupled reduction of ferricyanide ions to ferrocyanide ions results in a measurable electrochemical potential. This measurable zero-current potential is found to be Nernstian in nature and directly proportional to the logarithm values of L-Iactate concentration over the range of 2 × 10-5to 5 × 10-2M. The results indicate that immobilized lactate dehydrogenase can be incorporated into a system to detect L-Lactate acid in aqueous solutions.
    Zusätzliches Material: 3 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/bit.260191205
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  • 4
    Digitale Medien
    Digitale Medien
    ON THE STRUCTURE OF STEADY FLOW THROUGH DUAL-INTAKE ENGINE PORTS (1996)
    Chichester : Wiley-Blackwell
    International Journal for Numerical Methods in Fluids 23 (1996), S. 1085-1109 
    Details
    ISSN: 0271-2091
    Schlagwort(e): intake port ; dual intake ; CFD ; laser Doppler anemometry ; internal combustion engine ; cylinder ; turbulence ; steady flow ; Engineering ; Engineering General
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Maschinenbau
    Notizen: The influence of intake port design on the flow field in a dual-intake valve engine was investigated using computational fluid dynamics, in order to study the effect of inlet port design on the in-cylinder flow. A detailed 3D computational grid incorporating all the features of the Ford Zetec production engine inlet ports, valves and cylinder head was initially created and the flow structure modelled at 5 and 10 mm valve lifts under steady flow conditions. Comparisons of computational results with experimental data obtained by laser Doppler anemometry indicate that the flow characteristics have been predicted well in most regions. Flow generated by different intake port designs was also simulated by introducing air into the cylinder at different directions to the inlet valve axes and the effects of port deactivation, throttling and exhaust gas recirculation were examined. The implications of the results for intake port design are discussed.
    Zusätzliches Material: 13 Ill.
    Materialart: Digitale Medien
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  • 5
    Digitale Medien
    Digitale Medien
    Velocity characteristics of steady flow through a straight generic inlet port (1995)
    Chichester : Wiley-Blackwell
    International Journal for Numerical Methods in Fluids 21 (1995), S. 571-590 
    Details
    ISSN: 0271-2091
    Schlagwort(e): CDF ; predictions ; laser Doppler anemometry ; inlet port ; cylinder ; turbulence ; steady flow ; Engineering ; Engineering General
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Maschinenbau
    Notizen: This paper presents a combined experimental and computational study of the steady flow through an internal combustion engine inlet port. The port was of generic design with a straight centreline. The three-dimensional velocity and turbulence fields in the port and cylinder were simulated using a computational fluid dynamics programme. Laser sheet flow visualization and laser Doppler anemometry were also employed to investigate the flows and assess the predictions. The results show that a large-scale flow structure is created in the cylinder by the inlet jet and its interaction with the valve and cylinder walls. Both predictions and measurements show that the flow is strongly dependent on the valve lift but is not affected by the flow rate. Comparisons of the numerical predictions with the experimental data indicated that the mean flow features are accurately predicted in many parts of the flow field; some discrepancies are evident and stem primarily from the failure of the simulation to predict a small recirculation region in the port which affects the trajectory of the annular jet entering the cylinder. Calculations were also made without modelling the port shape by using simplified inlet conditions upstream of the valve seat. It was found that this approximation can provide a reasonable, albeit less accurate, description of the flow, but modelling of the port shape is necessary for accurate flow predictions.
    Zusätzliches Material: 18 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/fld.1650210704
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  • 6
    Digitale Medien
    Digitale Medien
    Raman and infrared study of reorientational dynamics in trifluorobenzene (1989)
    Chichester [u.a.] : Wiley-Blackwell
    Journal of Raman Spectroscopy 20 (1989), S. 27-30 
    Details
    ISSN: 0377-0486
    Schlagwort(e): Chemistry ; Analytical Chemistry and Spectroscopy
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Physik
    Notizen: The Raman spectral bandwidths of two A1′ vibrations of 1,3,5-trifluorobenzene (TFB) were measured in the neat liquid as a function of temperature and in the solvents CH2Cl2 and CS2. The Raman and IR linewidths of two E′ modes were also measured in solution. Derived values of the two rotational diffusion constants, D⊥ and D∥, were compared with the predictions of several current theories. It was observed that the Hynes-Kapral-Weinberg (HKW) theory is superior to both the microviscosity-free rotor and Hu-Zwanzig ‘slip’ models of reorientational diffusion. Calculated values of D⊥ and D∥ for TFB in solution using the HKW model are in excellent agreement with the experimentally measured diffusion constants.
    Zusätzliches Material: 4 Tab.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/jrs.1250200106
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  • 7
    Digitale Medien
    Digitale Medien
    Solvent dependence of 13C relaxation times and reorientational dynamics in tribromobenzene (1988)
    Chichester : Wiley-Blackwell
    Organic Magnetic Resonance 26 (1988), S. 675-678 
    Details
    ISSN: 0749-1581
    Schlagwort(e): Tribromobenzene ; 13C spin-lattice relaxation times ; correlation times ; Chemistry ; Analytical Chemistry and Spectroscopy
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie
    Notizen: The 13C spin-lattice relaxation times and nuclear Overhauser enhancements of protonated carbons in 1, 3, 5-tribromobenzene were measured in a number of solvents of widely varying viscosity, Derived reorientational correlation times were found to increase approximately linearly with solution viscosity, as predicted by hydrodynamic theories of rotational diffusion.Rotational correlation times calculated from the perrin stick model were two to three times longer than the measured τc values. Similarly, correlation times predicted by the Hu-Zwanzig slip model were too small by a factor of two. On the other hand, application of the newer Hynes-Kapral-Weinberg theory furnished reorientational correlation times that were in virtually quantitative agreement with the experimental results.
    Zusätzliches Material: 1 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/mrc.1260260807
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