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Specific detection of Helicobacter pylori and non–Helicobacter pylori flora in small- and large-cell primary gastric B-cell non-hodgkin's lymphoma (1997)

Gisbertz, I. A. M. ; Jonkers, D. M. A. E. ; Arends, J. W. ; [et al.]

Springer

Annals of oncology 8 (1997), S. 33-36

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ISSN: 1569-8041

Keywords: bacterial flora ; Helicobacter pylori ; immunohisochemistry ; MALT ; primary gastric lymphoma

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Abstract Background: Primary gastric non-Hodgkin's lymphomas possibly develop in response to local infection by Helicobacter pylori (H. pylori). We investigated the presence of H. pylori and non–H. pylori flora histologically in small- and large-cell primary gastric lymphoma using a specific staining method. Materials and methods: Specimens of 52 cases of primary gastric lymphoma(17 small cell, 35 large cell) were stained with modified Giemsa (MG) and immunohistochemically using a polyclonal antibody against H. pylori (IHC). Results: Thirty-two cases (61.5%) (small cell 76% versus large cell 53%,P 〉 0.05) showed immunoreactivity for H. pylori in the mucosa surrounding the tumor. Remarkable, there waslocalization of H. pylori in the neck of the gastric glands in 3 cases.Non– H. pylori flora was seen in 35 cases (76.3%) (small cell53% versus large cell 74%,P 〉 0.05). In 20cases, this non– H. pylori flora was mixed with H. pylori. Five cases showed no bacterial flora at all. Conclusions: (1)Using immunohistochemistry, the prevalence of gastriclymphoma cases with H. pylori (61.5%) approximates that of H. pylori in the normal population. (2) No statistical difference was found between the occurrence of H. pylori and non–H. pyloribacterial flora in small- versus large-cell lymphoma. (3) Our results suggest that H. pylori may not be the only etiologic factor in primary gastric lymphoma.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1008205510594

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Specific detection of Helicobacter pylori and non–Helicobacter pylori flora in small- and large-cell primary gastric B-cell non-hodgkin's lymphoma (1997)

Gisbertz, I. A. M. ; Jonkers, D. M. A. E. ; Arends, J. W. ; [et al.]

Springer

Annals of oncology 8 (1997), S. 33-36

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ISSN: 1569-8041

Keywords: bacterial flora ; Helicobacter pylori ; immunohisochemistry ; MALT ; primary gastric lymphoma

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Abstract Background: Primary gastric non-Hodgkin's lymphomas possibly develop inresponse to local infection by Helicobacter pylori (H. pylori). Weinvestigated the presence of H. pylori and non–H. pylori florahistologically in small- and large-cell primary gastric lymphoma using aspecific staining method. Materials and methods: Specimens of 52 cases of primary gastric lymphoma(17 small cell, 35 large cell) were stained with modified Giemsa (MG) andimmunohistochemically using a polyclonal antibody against H. pylori (IHC). Results: Thirty-two cases (61.5%) (small cell 76% versuslarge cell 53%,P 〉 0.05) showed immunoreactivityfor H. pylori in the mucosa surrounding the tumor. Remarkable, there waslocalization of H. pylori in the neck of the gastric glands in 3 cases.Non– H. pylori flora was seen in 35 cases (76.3%) (small cell53% versus large cell 74%,P 〉 0.05). In 20cases, this non– H. pylori flora was mixed with H. pylori. Five casesshowed no bacterial flora at all. Conclusions: (1)Using immunohistochemistry, the prevalence of gastriclymphoma cases with H. pylori (61.5%) approximates that of H. pylori in the normal population. (2) No statistical difference was foundbetween the occurrence of H. pylori and non–H. pyloribacterial flora in small- versus large-cell lymphoma. (3) Our resultssuggest that H. pylori may not be the only etiologic factor in primarygastric lymphoma.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1008205510594

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Calculation of the Raman line broadening on carbonation in synthetic hydroxyapatite (1988)

de Mul, F. F. M. ; Otto, C. ; Greve, J. ; [et al.]

Chichester [u.a.] : Wiley-Blackwell

Journal of Raman Spectroscopy 19 (1988), S. 13-21

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ISSN: 0377-0486

Keywords: Chemistry ; Analytical Chemistry and Spectroscopy

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology , Physics

Notes: The position and broadening of the Raman band associated with the phosphate symmetric stretching vibration in hydroxyapatite are simulated using a simple inter- and intra-ionic potential. The results are compared with experimental values. This comparison was made as a function of the incorporation of carbonate ions in the lattice for a number of substitution models. The line width of the phosphate symmetric stretching vibration is shown both theoretically and experimentally to be dependent on the carbonate content. Good agreement between calculations and experiment is obtained. The results of the calculations offer some support to the suggestions in the literature that the dielectric constant has to be considered as a function of the distance in the lattice, increasing beyond the first environmental layer.

Additional Material: 8 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/jrs.1250190104

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